Available software
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RELION
Single-particle processing package. Also includes MotionCor, CTFFIND and Gctf.
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Scipion
Image processing framework for obtaining 3D models of macromolecular complexes using Electron Microscopy (3DEM).
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EMAN
Greyscale scientific image processing suite with a primary focus on processing TEM data (single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography).
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cryoSPARC
Software platform for obtaining 3D structural information from single particle cryo-EM data.
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Sphire
Software suite designed for easy access to cryo electron microscopy with the clear goal of quality assessment and result reproducibility by statistical resampling.
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cisTEM
Processes cryo-EM images of macromolecular complexes and obtains high-resolution 3D reconstructions with tools to process image data including movies, micrographs and stacks of single-particle images, implementing a complete “pipeline” of processing steps to obtain high-resolution single-particle reconstructions.
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IMOD
Open-source, cross-platform suite of modeling, display and image processing programs used for 3D reconstruction and modeling of microscopy images with a special emphasis on electron microscopy data.
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Coot
Macromolecular model building, model completion and validation, particularly suitable for protein modelling using X-ray data.
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Chimera
Interactive visualization and analysis of molecular structures and related data, including density maps, supramolecular assemblies, sequence alignments, docking results, trajectories, and conformational ensembles.
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ChimeraX
Molecular visualization program
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Phenix
Python-based Hierarchical ENvironment for Integrated Xtallography
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