Dr Thierry Nouspikel

Non-Clinical Lecturer
 
Telephone: +44 (0)114 271 2966
Email:t.nouspikel@sheffield.ac.uk 
Institute for Cancer Studies
G Floor Medical School
Beech Hill Road
Sheffield S10 2RX
United Kingdom

Education:

1980......College Claparède, Geneva, Switzerland.  Baccalaureate (valedictorian).
1987......University of Geneva, School of Medicine. Diploma in medicine.
1992......University of Geneva, School of Medicine.  M.D. degree.
1996......University of Geneva, School of Sciences.  Ph.D. in biology
 

Positions and Employment:

1988-1992.....M.D. student, Dept of Clinical Biochemistry, University of Geneva. Dr Patrick B. Iynedjian.
1992-1996.....Ph.D. student, Dept of Genetics and Microbiology, University of Geneva. Prof Stuart G. Clakson.
1996-2000.....Post-doctoral fellow, Dept of Biological Sciences, Stanford University.Prof Philip C. Hanawalt
2001-2005.....Life Sciences Research Associate, Dept of Biological Sciences, Stanford University.
 

Principal sources of funding:

2006-2008  Leukaemia Research Fund project grant. Modulations of nucleotide excision repair in B lymphocytes at various stages of differentiation.

2006-2009   BBSRC new investigator grant. Characterizing the control of nucleotide excision repair by ubiquitination through differential E1 phosphorylation.

2009-2010    Yorkshire Cancer Research pump-priming grant. Measuring nucleotide excision repair and mutagenesis in cultured human embyonic stem cells.

Teaching:

MBB313 Genome Stability and Genetic Change (Module coordinator:  Dr A.S.H. Goldman)

Selected Publications:

DNA repair in differentiated cells

Nouspikel T. DNA repair in mammalian cells: So DNA repair really is that important? Cell Mol Life Sci. 66:965-7 (2009) Editorial.

Nouspikel T. DNA repair in mammalian cells : Nucleotide excision repair: variations on versatility. Cell Mol Life Sci. 66:994-1009 (2009). Review. [Abstract]

Nouspikel, T. Differentiated Cells Play Favorites: Dissecting the Mechanisms of Discrimination in DNA Repair. In: Progress in DNA Damage Research, Miura, S. and Nakano, S. Editors. Nova Science Publishers, Inc. (2008) Book Chapter. [Abstract]

Nouspikel T. Nucleotide excision repair and neurological diseases.DNA Repair 7:1155-67 (2008). Review.[Abstract]

Nouspikel, T. and Hanawalt, P.C. Impaired nucleotide excision repair upon macrophage differentiation is corrected by E1 ubiquitin-activating enzyme.Proc Natl Acad Sci USA. 103:16188-93 (2006) [Abstract] [Full paper].

Nouspikel, T. Hyka-Nouspikel, N., Hanawalt, P.C. Transcription domain-associated repair in human cells.Mol Cell Biol 26:8722-8730 (2006) [Abstract]

Nouspikel, T. DNA repair in differentiated cells: Some new answers to old questions.Neuroscience 145:1213-1221 (2007) [Abstract]

Hsu, P.H., Hanawalt, P.C., and Nouspikel, T. Nucleotide excision repair phenotype of human acute myeloid leukemia cell lines at various stages of differentiation. Mut Research 614:3-15 (2007) [Abstract]

Nouspikel,T. and Hanawalt, P.C. When parsimony backfires: neglecting DNA repair may doom neurons in Alzheimer's disease. BioEssays 25:168-173 (2003) [Abstract]

Nouspikel,T. and Hanawalt, P.C. DNA repair in terminally differentiated cells. DNA Repair 1:59-75 (2002) [Abstract]

Nouspikel, T., and Hanawalt, P.C. Terminally differentiated human neurons repair transcribed genes but display attenuated global DNA repair and modulation of repair gene expression. Mol. Cel. Biol. 20:1562-1570 (2000) [Abstract][Full paper]


Role of XPG in xeroderma pigmentosum and Cockayne syndrome

Thorel, F., Constantinou, A., Dunand-Sauthier, I., Nouspikel, T., Lalle, P., Raams, A., Jaspers, N.G., Vermeulen, W., Shivji, M.K., Wood, R.D., and Clarkson, S.G. Definition of a short region of XPG necessary for TFIIH interaction and stable recruitment to sites of UV damage.Mol Cell Biol. 24:10670-80 ( 2004) [Abstract][Full paper]

Lalle, P., Nouspikel, T., Constantinou, A., Thorel, F., and Clarkson, S.G. The founding members of xeroderma pigmentosum group G produce XPG protein with severely impaired endonuclease activity. J. Invest. Dermatol. 118:344-51 (2002) [Abstract]

Nouspikel, T., Lalle, P., Leadon, S.A., Cooper, P.K., and Clarkson S.G. A common mutational pattern in Cockayne syndrome patients  from xeroderma pigmentosum group G: Implications for a second XPG function. Proc. Natl. Acad. Sci. USA 94:3116-3121 (1997) [Abstract][Full paper]

Nouspikel, T. and Clarkson, S. G. Mutations that disable the DNA repair gene XPG in a xeroderma pigmentosum group G patient. Hum. Mol. Genet. 3:963-967 (1994) [Abstract]

Scherly, D., Nouspikel, T., Corlet, J., Ucla, C., Bairoch, A., and Clarkson, S. G. Complementation of the DNA repair defect in xeroderma pigmentosum group G cells by a human cDNA related to yeast RAD2. Nature 363:182-184 (1993) [Abstract]
 

Glucokinase in primary rat hepatocytes

Nouspikel,T. and Iynedjian, P. B. Insulin signaling and regulation of glucokinase gene expression in cultured hepatocytes. Eur. J. Biochem. 210:365-373 (1992) [Abstract]

Nouspikel,T., Gjinovci, A., Li, S., and Iynedjian, P. B. Unimpaired effect of insulin on glucokinase gene expression in hepatocytes challenged with amylin.  FEBS Lett. 301:115-118 (1992) [Abstract]

Iynedjian, P. B., Pilot, P.-R., Nouspikel, T., Milburn, J. L., Quaade, C., Hughes, S., Ucla, C., Newgard, C. B. Differential expression and regulation of the glucokinase gene in liver and islet of Langerhans.Proc. Natl. Acad. Sci. USA 86, 7838-7842 (1989) [Abstract] [Full paper]

Iynedjian, P. B., Jotterand, D., Nouspikel, T., Asfari, M., and Pilot, P.-R. Transcriptional induction of glucokinase gene by insulin in cultured liver cells and its repression by the glucagon-cAMP system. J. Biol. Chem. 264, 21824-21829 (1989) [Abstract][Full paper]

Research Group:



From left to right:

Research Interests:

I am interested in DNA repair, particularly in the Nucleotide Excision Repair pathway, and in its modulation during cellular differentiation. Since the latter appears to be controlled by ubiquitination, I am becoming increasingly interested in the regulation of ubiquitination.

See the lab web page for more details.

Vacant Positions:

None at this time.