Prof. Stuart J. Edelstein: Single Binding Events

The multiple transitions between conformational states in single binding life-times.

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For additional details see:

Edelstein, S.J., Schaad, O. and Changeux, J.P. (1997). Single binding versus single channel recordings: a new approach to study ionotropic receptors. Biochemistry 36, 13755-13760. [Abstract]

single binding dwell-time profiles

The simulations were conducted for muscle nicotinic acetylcholine receptors with two non-equivalent sites and two conformational states, B (closed ion channel, low afinity) and A (open ion channel, high affinity) . All calculations were based on a ligand concentration of 2 x 10-5 M. For the species with one molecule of agonist bound to the high affinity site "H" the individual dwell time profiles above are presented for binding of a second molecule, with single binding events prolonged by incresing numbers of passages between B2 and A2.

	The multiple transitions between conformational states in single binding life-times.
RETURN TO RESEARCH ACTIVITIES For additional details see: Edelstein, S.J., Schaad, O. and Changeux, J.P. (1997). Single binding versus single channel recordings: a new approach to study ionotropic receptors. Biochemistry 36, 13755-13760. [Abstract]
	The simulations were conducted for muscle nicotinic acetylcholine receptors with two non-equivalent sites and two conformational states, B (closed ion channel, low afinity) and A (open ion channel, high affinity) . All calculations were based on a ligand concentration of 2 x 10-5 M. For the species with one molecule of agonist bound to the high affinity site "H" the individual dwell time profiles above are presented for binding of a second molecule, with single binding events prolonged by incresing numbers of passages between B2 and A2.