The junctional proteins cingulin and paracingulin modulate the expression of tight junction protein genes through GATA-4.

Guillemot, L., Spadaro, D., Citi, S.

PLoS ONE (2013) 8 (2): e55873. doi10.1371/journal/pone.0055873

Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.

Guillemot, L., Schneider, Y., Brun, P., Castagliuolo, I., Pizzuti, D., Martines, D., Jond, L., Bongiovanni, M., Citi, S.

  1. J.Cell Sci. (2012) 125: 5005-5014.

Distinct domains of paracingulin are involved in its targeting to the actin cytoskeleton and regulation of apical junction assembly.

Paschoud, S., Guillemot, L, Citi, S.

J. Biol. Chem. (2012) 287(16): 13159-13169.

The control of gene expression and cell proliferation by the epithelial apical junctional complex.

Spadaro, D., Tapia, R., Pulimeno, P., Citi, S.

Essays in Biochemistry (2012) 53:83-93.

Cingulin, paracingulin and PLEKHA7: signalling and cytoskeletal adaptors at the apical junctional complex.

Citi, S., Pulimeno P., Paschoud S.

Ann. NY Acad. Sci (2012) 1257:125-132.

Regulation of small GTPases at epithelial cell-cell junctions.

Citi, S., Spadaro, D., Schneider, Y., Stutz J. Pulimeno P.

Mol. Membr. Biol. (2011) 28:427-444.

Cingulin and paracingulin show similar dynamic behaviour, but are recruited independently to junctions.

Paschoud, S., Yu, D., Pulimeno, P., Jond, L., Turner, J.R, Citi. S.

Mol. Membr. Biol. (2011) 28:123-135

A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells.

Pulimeno, P., Paschoud. S., Citi, S.

J. Biol. Chem. (2011) 286(19): 16743-50.


PLEKHA7 is an adherens junction protein with a tissue distribution and subcellular localization distinct from ZO-1 and E-cadherin.

Pulimeno, P., Bauer, C., Stutz, J., Citi, S.

PLoS ONE (2010) 5(8): e12207

The tight junction protein cingulin regulates gene expression and RhoA signaling.

Citi S., Paschoud S., Pulimeno P., Timolati F., De Robertis F., Jond L., Guillemot L.

Ann. NY Acad. Sci. (2009) 1165:88-98

Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression.

Paschoud, S., Citi, S.

Mol. Membr. Biol (2008) 25:1-13

Paracingulin regulates the activity of Rac1 and RhoA GTPases by recruiting Tiam1 and GEF-H1 to epithelial junctions.

Guillemot, L., Paschoud, S., Jond, L., Foglia, A., Citi, S.

Mol. Biol. Cell (2008) 19:4442-4453

The cytoplasmic plaque of tight junctions: A scaffolding and signalling center.

Guillemot, L., Foglia, A., Paschoud, S., Pulimeno, P., Citi, S. 

Biochim Biophys Acta (2008) 1778(3):601-13.


The adherens junctions control susceptibility to Staphylococcus aureus a-toxin

Popov, L.M., Marceau, C.,D., Starkl, P.M., Lumb, J.H., Shah, J., Guerrera, D., Cooper, R.L., Merakou, C., Bouley, D.M., Meng, W., , Kiyonari, H., Takeichi, M., Galli, S.J., Bagnoli, F., Citi, S., Carette, J.E., Amieva M.R.

Proc. Nat. Acad. Sci USA. (2015) 112: 14337-42. doi:10.1073/pnas.1510265112

Distinct cell-cell adhesion complexes regulate cell behavior either by engaging RNAi or via Src-p120 activity

Kourtidis A., Ngok, S.P., Pulimeno, P., Feathers, R.W., Carpio, L.R., Baker, T.R., Carr, J.M., Dachsel J.C., Perez, E.A., Storz, P., Copland, J.A., Thompson, E.A.,

Citi, S., Anastasiadis, P.Z.

Nature Cell Biol. (2015) 17: 1145-57. doi:10.1038/ncb3227

The expression of the zonula adhaerens protein PLEKHA7 is strongly decreased in high grade ductal and lobular breast carcinomas

Tille, J.C., Ho, L., Shah, J., Seyde, O., McKee, T.A., Citi, S.

PLoS One (2015) 10(8):e0135442. doi: 10.1371/journal.pone.0135442

Epithelial junctions and Rho family GTPases: the zonular signalosome

Citi, S., Guerrera, D., Spadaro, D., Shah, J.

Small GTPases (2014) DOI:10.4161/21541248.2014.97376036382. Link

Link to Research Gate full-text

ZO proteins redundantly regulate the transcription factor DbpA/ZONAB

Spadaro, D., Tapia, R., Jond, L., Sudol, M., Fanning, A.S., Citi, S.

J. Biol. Chem (2014) 289:22500-22511. Link

Toll-like receptor 2 regulates the barrier function of human bronchial epithelial monolayers through atypical protein kinase C zeta, and an increase in expression of claudin-1

Ragupathy, S., Esmaeili, F., Paschoud, S., Sublet, E., Citi, S., Borchard, G.

Tissue Barriers (2014), 2: e29166. Link

MgcRacGAP interacts with cingulin and paracingulin to regulate Rac1 activation and development of the tight junction barrier during epithelial junction assembly

Guillemot, L., Guerrera, D., Spadaro, D., Tapia, R., Jond, L., Citi, S.

Molecular Biology of the Cell (2014) mbc.E13-11-0680. Link

PLEKHA7 modulates epithelial tight junction barrier function

Paschoud, S., Jond, L., Guerrera, D., Citi, S.

Tissue Barriers (2014) 2:e28755. Link


Tension-dependent stretching activates ZO-1 to control the junctional localization of its interactors

Spadaro, D., Le, S., Laroche, T., Mean, I., Jond, L., Yan, J., Citi, S.

Current Biology (2017) 27:1-13; DOI:

Cell-specific diversity in the expression and organization of cytoplasmic plaque proteins of apical junctions

Vasileva, E., Sluysmans, S., Bochaton-Piallat, M-L., Citi, S. ,

Ann. N.Y. Acad. Sci (2017) Jun. 15; doi: 10.1111/nyas.13391. (Epub ahead of print)

The role of apical cell-cell junctions and associated cytoskeleton in mechanotransduction

Sluysmans, S., Vasileva, E., Spadaro, D., Shah, J., Rouaud, F., Citi, S. ,

Biol. Cell. (2017) Feb. 21; doi: 10.1111/boc.201600075 (Epub ahead of print). Review

Cingulin role in agonist-induced vascular endothelial permeability

Tian, Y., Gawlak, G., Tian, X., Shah, A.S., Sarich, N., Citi, S. , Birukova, A.A.

J. Biol. Chem. (2016) Sept. 02; pii: jbc.M116.720763 (Epub ahead of print)

Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells

Mangan, A. J., Sietsema, D.V., Li, D., Moore, J.K., Citi, S., Prekeris R.

Nature Comm. (2016) doi:10.1038/ncomms12426 (Epub 2016 Aug 3)

PLEKHA7 recruits PDZD11 to adherens junctions to stabilize nectins

Guerrera, D., Shah, J., Vasileva, E., Sluysmans, S., Méan, I., Jond, L.,  Poser, I., Mann, M., Hyman, A.A., Citi, S.

J. Biol. Chem. (2016) May 20; 291 (21): 11016-29. doi: 10.1074/ jbc.M115.712935 (Epub 2016 Apr 4)

Grete Kellenberger-Gujer: Molecular biology research pioneer

Citi, S., Berg. D.E

Bacteriophage (2016), 6:2, e1173168, DOI: 10.1080/21597081.2016.1173168

PLEKHA7: cytoskeletal adaptor protein at center stage in junctional organization and signaling

Shah, J., Guerrera, D., Vasileva, E., Sluysmans, S., Bertels, E., Citi, S.

Int. J. Biochem. Cell Biol. (2016) 75: 112-116

Evidence that cingulin regulates endothelial barrier function in vitro and in vivo

Schlossleitner, K., Rauscher, S., Gröger M., Friedl, H.P., Finsterwalde, R., Habertheuer, A., Sibilia, M., Brostjan, C., Födinger, D., Citi, S., Petzelbauer, P.

Arterioscler. Thromb. Vasc. Biol. (2016) 36: 647-654


Intestinal barriers protect against disease

Citi, S.

Science (2018) 359: 1097-1098.