Plant Biochemistry and Physiology

Publications in peer reviewed scientific journals
Book chapters
Press Releases

Publications in peer reviewed scientific journals

Coquille S., Roux C., Fitzpatrick T.B. & Thore S.: The last piece in the vitamin B1 biosynthesis puzzle: Structural and functional insight into yeast HMP-P synthase.
J Biol Chem 2012. PMID: 23048037.

Fitzpatrick T.B., Basset G.J., Borel P., Carrari F., Dellapenna D., Fraser P.D., Hellmann H., Osorio S., Rothan C., Valpuesta V., Caris-Veyrat C. & Fernie A.R.: Vitamin deficiencies in humans: can plant science help?
Plant Cell 2012, 24(2):395-414. PMID: 22374394.

Raschke M., Boycheva S., Crevecoeur M., Nunes-Nesi A., Witt S., Fernie A.R., Amrhein N. & Fitzpatrick T.B.: Enhanced levels of vitamin B(6) increase aerial organ size and positively affect stress tolerance in Arabidopsis.
Plant J 2011, 66(3):414-432. PMID: 21241390.

Moccand C., Kaufmann M. & Fitzpatrick T.B.: It takes two to tango: defining an essential second active site in pyridoxal 5'-phosphate synthase.
PLoS One 2011, 6(1):e16042. PMID: 21283685.

Fitzpatrick T.B., Moccand C. & Roux C.: Vitamin B6 biosynthesis: charting the mechanistic landscape.
Chembiochem 2010, 11(9):1185-1193. PMID: 20397182.

Raschle T., Speziga D., Kress W., Moccand C., Gehrig P., Amrhein N., Weber-Ban E. & Fitzpatrick T.B.: Intersubunit cross-talk in pyridoxal 5'-phosphate synthase, coordinated by the C terminus of the synthase subunit.
J Biol Chem 2009, 284(12):7706-7718. PMID: 19074821.

Benabdellah K., Azcon-Aguilar C., Valderas A., Speziga D., Fitzpatrick T.B. & Ferrol N.: GintPDX1 encodes a protein involved in vitamin B6 biosynthesis that is up-regulated by oxidative stress in the arbuscular mycorrhizal fungus Glomus intraradices.
New Phytol 2009, 184(3):682-693. PMID: 19674326.

Tambasco-Studart M., Tews I., Amrhein N. & Fitzpatrick T.B.: Functional analysis of PDX2 from Arabidopsis, a glutaminase involved in vitamin B6 biosynthesis.
Plant Physiol 2007, 144(2):915-925. PMID: 17468224.

Raschle T., Arigoni D., Brunisholz R., Rechsteiner H., Amrhein N. & Fitzpatrick T.B.: Reaction mechanism of pyridoxal 5'-phosphate synthase: detection of an enzyme-bound chromophoric intermediate.
J Biol Chem 2007, 282(9):6098-6105. PMID: 17189272.

Raschke M., Burkle L., Muller N., Nunes-Nesi A., Fernie A.R., Arigoni D., Amrhein N. & Fitzpatrick T.B.: Vitamin B1 biosynthesis in plants requires the essential iron sulfur cluster protein, THIC.
Proc Natl Acad Sci U S A 2007, 104(49):19637-19642. PMID: 18048325.

Fitzpatrick T.B., Amrhein N., Kappes B., Macheroux P., Tews I. & Raschle T.: Two independent routes of de novo vitamin B6 biosynthesis: not that different after all.
Biochem J 2007, 407(1):1-13. PMID: 17822383.

Titiz O., Tambasco-Studart M., Warzych E., Apel K., Amrhein N., Laloi C. & Fitzpatrick T.B.: PDX1 is essential for vitamin B6 biosynthesis, development and stress tolerance in Arabidopsis.
Plant J 2006, 48(6):933-946. PMID: 17227548.

Strohmeier M., Raschle T., Mazurkiewicz J., Rippe K., Sinning I., ^§Fitzpatrick T.B. & ^§Tews I.: Structure of a bacterial pyridoxal 5'-phosphate synthase complex.
Proc Natl Acad Sci U S A 2006, 103(51):19284-19289. PMID: 17159152.
(^§ co-corresponding authors)

Gengenbacher M., Fitzpatrick T.B., Raschle T., Flicker K., Sinning I., Muller S., Macheroux P., Tews I. & Kappes B.: Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights.
J Biol Chem 2006, 281(6):3633-3641. PMID: 16339145.

Tambasco-Studart M., Titiz O., Raschle T., Forster G., Amrhein N. & Fitzpatrick T.B.: Vitamin B6 biosynthesis in higher plants.
Proc Natl Acad Sci U S A 2005, 102(38):13687-13692. PMID: 16157873.

Raschle T., Amrhein N. & Fitzpatrick T.B.: On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis.
J Biol Chem 2005, 280(37):32291-32300. PMID: 16030023.

Morokutti A., Lyskowski A., Sollner S., Pointner E., Fitzpatrick T.B., Kratky C., Gruber K. & Macheroux P.: Structure and function of YcnD from Bacillus subtilis, a flavin-containing oxidoreductase.
Biochemistry 2005, 44(42):13724-13733. PMID: 16229462.

Kitzing K., Fitzpatrick T.B., Wilken C., Sawa J., Bourenkov G.P., Macheroux P. & Clausen T.: The 1.3 Å crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.
J Biol Chem 2005, 280(30):27904-27913. PMID: 15890652.

McConkey G.A., Pinney J.W., Westhead D.R., Plueckhahn K., Fitzpatrick T.B., Macheroux P. & Kappes B.: Annotating the Plasmodium genome and the enigma of the shikimate pathway.
Trends Parasitol 2004, 20(2):60-65. PMID: 14747018.

Fitzpatrick T.B., Auweter S., Kitzing K., Clausen T., Amrhein N. & Macheroux P.: Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.
Protein Expr Purif 2004, 36(2):280-291. PMID: 15249051.

Fitzpatrick T.B., Amrhein N. & Macheroux P.: Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response.
J Biol Chem 2003, 278(22):19891-19897. PMID: 12660247.

Geyer A., Fitzpatrick T.B., Pawelek P.D., Kitzing K., Vrielink A., Ghisla S. & Macheroux P.: Structure and characterization of the glycan moiety of L-amino-acid oxidase from the Malayan pit viper Calloselasma rhodostoma.
Eur J Biochem 2001, 268(14):4044-4053. PMID: 11453999.

Fitzpatrick T.B., Killer P., Thomas R.M., Jelesarov I., Amrhein N. & Macheroux P.: Chorismate synthase from the hyperthermophile Thermotoga maritima combines thermostability and increased rigidity with catalytic and spectral properties similar to mesophilic counterparts.
J Biol Chem 2001, 276(21):18052-18059. PMID: 11279147.

Fitzpatrick T., Ricken S., Lanzer M., Amrhein N., Macheroux P. & Kappes B.: Subcellular localization and characterization of chorismate synthase in the apicomplexan Plasmodium falciparum.
Mol Microbiol 2001, 40(1):65-75. PMID: 11298276.

O'Sullivan D.B., O'Connell T.P., Mahon M.M., Koenig A., Milne J.J., Fitzpatrick T.B. & Malthouse J.P.: ¹³C NMR study of how the oxyanion pKa values of subtilisin and chymotrypsin tetrahedral adducts are affected by different amino acid residues binding in enzyme subsites S1-S4.
Biochemistry 1999, 38(19):6187-6194. PMID: 10320347.

Mahon M.M., Gramatikova S.I., Christen P., Fitzpatrick T.B. & Malthouse J.P.: The pyridoxal-5'-phosphate-dependent catalytic antibody 15A9: its efficiency and stereospecificity in catalysing the exchange of the alpha-protons of glycine.
FEBS Lett 1998, 427(1):74-78. PMID: 9613603.

Fitzpatrick T.B. & Malthouse J.P.: A substrate-induced change in the stereospecificity of the serine-hydroxymethyltransferase-catalysed exchange of the alpha-protons of amino acids: evidence for a second catalytic site.
Eur J Biochem 1998, 252(1):113-117. PMID: 9523719.

Fitzpatrick T.B. & Malthouse J.P.: The effect of histidine-228 on the catalytic efficiency and stereospecificity of the serine hydroxymethyltransferase catalysed exchange of the alpha-protons of amino acids.
Biochim Biophys Acta 1998, 1386(1):220-226. PMID: 9675289.

Malthouse J.P., Fitzpatrick T.B., Milne J.J., Grehn L. & Ragnarsson U.: Enzymatic synthesis of isotopically labelled serine and tryptophan for application in peptide synthesis.
J Pept Sci 1997, 3(5):361-366. PMID: 9391911.

Book chapters

Fitzpatrick, T.B., Amrhein, N. & Macheroux, P. Vitamin B6 in plants: More than meets the eye.
Advances in Botanical research: Biosynthesis of vitamins in plants 2011 (Series edited by Kader J-C. and Delseny M.; Volume edited by Rébeillé F. and Douce R.) Academic Press, vol. 59, pp 1-38. ISBN: 978-0-12-385853-5

Fitzpatrick, T.B., Amrhein, N. & Macheroux, P. Characterisation of an Old Yellow Enzyme homologue from Bacillus subtilis.
Flavins and Flavoproteins 2002 (edited by Perham, R.N., Chapman S.K. & Scrutton, N.S.) 993-998, Weber (Berlin)

Fitzpatrick, T.B., Amrhein, N. & Macheroux, P. Analysis of a trimeric complex involving chorismate synthase from Bacillus subtilis.
Flavins and Flavoproteins 1999 (edited by Ghisla, S., Kroneck, P., Macheroux, P. and Sund, H.) 749-752, Weber (Berlin)

Press Releases

“Karriereschub dank Vitamin B6” ETH life. 11/2008

“Ein lebenswichtiges Vitamin in Pflanzen” ETH Globe 09/2008

“Important piece of the jigsaw found” ETH life. 04/12/2007

“Wichtiges Puzzleteil gefunden” ETH life. 04/12/2007

“Now it’s a question of details” ETH life. 29/03/2007

“Nun geht es um die Details” ETH life. 20/03/2007

Zahnrad produziert Vitamin B6. ETH life. 11/12/2006

Pflanzen machen es anders. ETH life. 13/10/2005

Neuer Produktionsweg für Vitamin B6. Neue Zürcher Zeitung. 16/11/2005