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HPLC calculator : software for chromatographic performance evaluation and HPLC method transfer
Licence : freeware |
date added : 22nd April, 2009 |
Version : 3 |
Downloaded 5173 times
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HPLC Calculator v3 (English)
Compatible system : Excel 97/2000/XP/2003
No install is necessary, only open the file "HPLC_calculator_version_ 3.xls.zip" with Excel.
Download (1.83 Mo)If you use this program, thanks to cite the following references:
- D. Guillarme, D. Nguyen, S. Rudaz, J.L. Veuthey, Eur. J. Pharm. Biopharm., 2007, 66, 475-482. (PDF)
- D. Guillarme, D. Nguyen, S. Rudaz, J.L. Veuthey, Eur. J. Pharm. Biopharm., 2008, 68, 430-440. (PDF)
Tutorial video (English)Compatible system: web navigator with flash plug-in.
Simply decompress the .zip archive in a folder and open the tutorial video with your favorite internet browser, equiped with a flash plug-in.
Download (2.04 Mo)
Guidelines for method transfer in HPLC
"HPLC calculator" is a tool developed at the LCAP to improve your HPLC analytical methods in terms of analysis time and/or resolution. It is not an optimization software and will not find the mobile phase conditions necessary to separate compounds of interest. Only kinetic parameters are evaluated.
1. The calculator determines chromatographic performance (i.e. efficiencies and induced backpressures) of 9 selected chromatographic columns, from Agilent, Waters and Thermo-Electron and gives the best conditions to be used in isocratic and gradient mode, according to the selected mobile and stationary phases as well as the analyte's molecular weight.
2. Furthermore, the calculator allows to transfer isocratic or gradient LC methods thanks to fundamental equations of chromatography. For gradient transfer, the system dwell volume should be indicated for calculation.
"HPLC calculator" could be used for any transfer between HPLC systems with different column geometries, between HPLC and UHPLC (or any other sub-2µm configurations) (down-scaling), but also from analytical LC to preparative LC (up-scaling).
Download (450.47 Ko)