Nikon Biostation IM
The Nikon BioStation IM incorporates a microscope, an incubator and a sensitive cooled CCD camera in a compact body. This all-in-one package provides a stable environment for live cells and advanced solutions for simple long-term time-lapse data acquisition.
Moreover, focus drift caused by thermal change and mechanical instability has been minimized, and fail-proof data acquisition is possible even during lengthy timelapse imaging. The system is user-friendly and easy to use.
Equipment and configuration
- Inverted microscope
- Incubator (5% CO2, 37°C)
- Objectives: dry: 40x (20x, 40x, 80x by switching magnification tube lens), NA 0.8
- Semrock filter blocs:
> GFP: EX 450-490/DM 495/BA 500-545
> YFP: EX 480-415/DM 520/BA 525-560
> TxRed: EX 540-580/DM595/BA 600-650
- Illumination: Intensilight precentered fiber mercury lamp
- Red LED illumination is used for phase contrast imaging (to reduce cell damage)
Applications
- Designed for live cell imaging
- 2D, 3D (Z stacks), multipositions, timelaps
- Acquisition in constant condition
- Phase contrast
- Fluorescence
- Perfusion during acquisition
- 1x 35 mm glass bottom dish, single or multichamber
(4 different conditions) or 1x slide