Nikon Biostation IM

The Nikon BioStation IM incorporates a microscope, an incubator and a sensitive cooled CCD camera in a compact body. This all-in-one package provides a stable environment for live cells and advanced solutions for simple long-term time-lapse data acquisition.
Moreover, focus drift caused by thermal change and mechanical instability has been minimized, and fail-proof data acquisition is possible even during lengthy timelapse imaging. The system is user-friendly and easy to use.

Equipment and configuration
  • Inverted microscope
  • Incubator (5% CO2, 37°C)
  • Objectives: dry: 40x (20x, 40x, 80x by switching magnification tube lens), NA 0.8
  • Semrock filter blocs:

> GFP: EX 450-490/DM 495/BA 500-545
> YFP: EX 480-415/DM 520/BA 525-560
> TxRed: EX 540-580/DM595/BA 600-650

  • Illumination: Intensilight precentered fiber mercury lamp
  • Red LED illumination is used for phase contrast imaging (to reduce cell damage)

  • Designed for live cell imaging
  • 2D, 3D (Z stacks), multipositions, timelaps
  • Acquisition in constant condition
  • Phase contrast
  • Fluorescence
  • Perfusion during acquisition
  • 1x 35 mm glass bottom dish, single or multichamber
    (4 different conditions) or 1x slide