Microscopic view of biological tissue

Harmonic Nanoparticles

The term Harmonic Nanoparticles (HNPs) designates a family of metal oxide nanocrystals possessing appealing optical properties aside from classical luminescence. In fact, because of the lack of inversion symmetry in their crystal structure, these materials display high nonlinear optical response. In particular, the lowest nonlinear term of their nonlinear susceptibility, Χ(2), is non vanishing differently from the case of isotropic materials. For this reason, they are primarily employed in imaging applications based on second harmonic generation. We have recently shown that third and even fourth harmonic generation are also very efficient for some of these materials, hence the name.

The distinctive features of HNPs as opposed to luminescent ones include their fully coherent emission, the absence of bleaching/blinking, and spectral flexibility (spanning from the ultraviolet to the infrared). This last asset is particularly appealing for bio-imaging applications, as HNPs allows imaging beyond the “classical” tissue transparency window (NIR I: 650-950 nm), in the newly investigated NIR II (1100-1350 nm) and NIR III (1600-1870 nm) windows. Working in these spectral regions enables sensibly deeper imaging penetration because of reduced scattering at longer wavelengths.

We demonstrated that the simultaneous detection of multiple harmonics from the same nano-object greatly benefit to selectivity for imaging and sensing. Our present research spans from bio-oriented applications (cell tracking in tissue) to more fundamental studies focused on the interplay among susceptibility tensors at different nonlinear orders.

Further readings

Multi-harmonic Imaging in the Second Near-Infrared Window of Nanoparticle-Labeled Stem Cells as a Monitoring Tool in Tissue Depth, L. Dubreil, I. Leroux, M. Ledevin, C. Schleder, L. Lagalice, C. Lovo, R. Fleurisson, S. Passemard, V. Kilin, S. Gerber-Lemaire, M.-A. Colle, L. Bonacina, and K. Rouger, ACS Nano (2017) [DOI: 10.1021/acsnano.7b00773]
Multi-Order Investigation of the Nonlinear Susceptibility Tensors of Individual Nanoparticles, C. Schmidt, J. Riporto, A. Uldry, A. Rogov, Y. Mugnier, R. Le Dantec, J.-P. Wolf and L. Bonacina, Sci. Rep. 6, 25415, (2016) [DOI: 10.1038/srep25415]
Simultaneous Multiharmonic Imaging of Nanoparticles in Tissues for Increased Selectivity, A. Rogov, M. Irondelle, F. Ramos Gomes, J. Bode, D. Staedler, S. Passemard, S. Courvoisier, Y. Yamamoto, F. Waharte, D. Ciepielewski, P. Rideau, S. Gerber-Lemaire, F. Alves, J. Salamero, L. Bonacina, and J.-P. Wolf, ACS Photonics, (2015) [DOI: 10.1021/acsphotonics.5b00289]
Harmonic nanoparticles: noncentrosymmetric metal oxides for nonlinear optics, A. Rogov Y. Mugnier and L. Bonacina J. Opt., 17 033001 (2015) [DOI: 10.1088/2040-8978/17/3/033001]
Nonlinear Nanomedecine: Harmonic Nanoparticles toward Targeted Diagnosis and Therapy, L. Bonacina, Mol. Pharmaceutics (2012) [DOI: 10.1021/mp300523e]
Harmonic Nanocrystals for Biolabeling: A Survey of Optical Properties and Biocompatibility, D. Staedler, T. Magouroux, R. Hadji, C. Joulaud, J. Extermann, S. Schwung, S. Passemard, C. Kasparian, G. Clarke, M. Gerrmann, R. Le Dantec, Y. Mugnier, D. Rytz, D. Ciepielewski, C. Galez, S. Gerber-Lemaire, L. Juillerat-Jeanneret, L. Bonacina, and J.-P. Wolf, ACS Nano, 6 (3), 2542–2549 (2012) [DOI: 10.1021/nn204990n]