LSM700 (Tel. 95 583)

Capabilities    Confocal_LSM700.pdf

• Confocal Laser Scanning Microscopy (CLSM)
• Imaging UV and VIS fluorophores (eg Dapi, YFP, CFP, GFP, TxRed, Alexa 488/568/633)
• Ideal for fixed specimens (oil objectives)
• 3D Z-stacks
• Colocalization studies

Detailed specifications

Microscope

• Zeiss Axio Imager.M2 microscope (upright)
• HXP120C fluorescence lamp for widefield eyepiece visualization

Objectives

"Plan-Neofluar" 10X / 0.30 "Plan-Neofluar" 20X / 0.50 "Plan-Neofluar" 40X / 1.3 Oil "Plan-Apochromat" 63x /1.4 Oil

Conventional mounted filters 

• UV excitation DAPI,Hoechst 33258, ...
  BP 365/12, FT395, LP397
• BLUE excitation FITC, GFP, YFP, CFP,...
  BP 450-490, FT 510, BP 515-565
• GREEN excitation TRITC, Texas-Red, DsRed1,...
  BP 546/12, FT 560, BP 575-640

Lasers

• 405 nm 5mW
• 488nm 10mW
• 555nm 10 mW
• 639 nm 5 mW

Detectors

• 2 reflection/fluorescence detection channels, each with highly sensitive PMT detectors, prepared for lasers of lasers of wavelengths 405, 488, 555 and 639 nm

Software

• Windows 7 64bits
• Zen 2010b version service pack1