Confocal Microscopy (5 systems)

LSM700 (Tel. 95 583)

Capabilities    Confocal_LSM700.pdf

  • Confocal Laser Scanning Microscopy (CLSM)
  • Imaging UV and VIS fluorophores (eg Dapi, YFP, CFP, GFP, TxRed, Alexa 488/568/633)
  • Ideal for fixed specimens (oil objectives)
  • 3D Z-stacks
  • Colocalization studies
LSM700_400.jpg

Detailed specifications

Microscope

  • Zeiss Axio Imager.M2 microscope (upright)
  • HXP120C fluorescence lamp for widefield eyepiece visualization

Objectives

  • "Plan-Neofluar" 10X / 0.30
  • "Plan-Neofluar" 20X / 0.50
  • "Plan-Neofluar" 40X / 1.3 Oil
  • "Plan-Apochromat" 63x /1.4 Oil
LSM700-10x.png LSM700-20x.png LSM700-40x.png LSM700-63x.png

Conventional mounted filters 

  • UV excitation DAPI,Hoechst 33258, ...
    BP 365/12, FT395, LP397
  • BLUE excitation FITC, GFP, YFP, CFP,...
    BP 450-490, FT 510, BP 515-565
  • GREEN excitation TRITC, Texas-Red, DsRed1,...
    BP 546/12, FT 560, BP 575-640

Lasers

  • 405 nm 5mW
  • 488nm 10mW
  • 555nm 10 mW
  • 639 nm 5 mW

Detectors

  • 2 reflection/fluorescence detection channels, each with highly sensitive PMT detectors, prepared for lasers of lasers of wavelengths 405, 488, 555 and 639 nm

Software

  • Windows 7 64bits
  • Zen 2010b version service pack1