Confocal Microscopy (4 systems)

Nikon A1r Spectral + Temp Controller + CO2 (Tel. 94 784)

capabilities  Confocal_Nikon_a1R_Spectral.pdf

  • Imaging live (short time-lapses < 2hours) and fixed samples- any fluorophores
  • Temperature
  • 3D/4D, colocalization, F-techniques (FRAP, FRET,...)
  • Spectral unmixing and spectral deconvolution
  • Multiposition
  • Tiled images

A1r_spectral_500.jpg

detailed specifications

Microscope

  • Nikon A1r+ microscope (Ti Eclipse inverted stand)
  • Resonant and Galvano scanner for fast imaging (up to 30 fps) and conventional imaging (15 fps), respectively
  • Motorized XY stage
  • Piezo
  • DIC prisme
  • Perfect Focus System 3 (PFS 3):

PSF detail.png

Objectives

  • 10x 0.45 CFI Plan Apochromat Lambda WD:4 mm
  • 20x 0.5 CFI Plan Fluor WD:2.1mm
  • 20x 0.45 CFI S Plan Fluor ELWD WD:8.2 -6.9mm correction:0-2mm
  • 40x 1.3 CFI Plan Fluor DIC WD:0.2mm
  • 40x 0.6 CFI ELWD S Plan Fluor WD:3.6-2.8mm correction:0-2mm
  • 60x 1.4 CFI Plan Apo Lambda WD:0.13mm

Mounted filters

Filter cubes for DAPI, CFP, eGFP, RedFP and DIC analyzer

Laser lines

  • 402 nm: 100 mW
  • 457 nm: 50 mW
  • 471 nm: 50 mW
  • 488 nm: 50 mW
  • 514 nm: 50 mW
  • 561 nm: 50 mW
  • 639 nm: 40 mW

Detectors

  • 4 PMTS including 2 GaAsp
  • 1 multispectral (spectral ranges from 32 channels with a sampling of 2.5, 6.0 or 10.0 nm)
  • 1 transmitted

Computer

  • Windows 7 64 bits
  • NIS Elements AR V 5.11.02