Nikon A1r + Temp Controller + CO2 (Tel. 94 783)
Capabilities Confocal_NikonA1r.pdf
- Imaging live samples- any fluorescent proteins
- Temperature, humidity, CO2 control
- Peristaltic pump, water bath
- Colocalization, F-techniques (FRAP, FRET, ...)
- Multiposition time-lapse
- Tiled images
- Optogenetic
Detailed specifications
Microscope
- Nikon A1r microscope (inverted)
- Resonant scanner for fast imaging up to 30 fps
- DIC prisme
- Motorised XY stage
- Perfect focus sytem (PFS):
Objectives
- 10x 0.45 CFI Plan Apochromat WD:4mm (MRD00101)
- 20x 0.45 CFI ELWD Plan Fluor WD:8.2-6.9mm correction:0-2mm (MRH08220)
- 20x 0.75 CFI Plan Apochromat VC WD:1mm (MRD70200)
- 40x 0.6 CFI ELWD S Plan Fluor WD:3.6-2.8mm correction 0-2mm (MRH08430)
- 40x 1.3 CFI Plan Fluor WD:0.2mm (MRH01401)
- 60x 1.4 CFI Plan Apochromat VC WD:0.13mm (MRD01602)
And also into the cupboard : 100x 1.4 CFI Plan Apochromat VC WD:0.13mm (MRD01901)
| Objective | Micron/ pixel value |
| 20x zoom 1 | 1.24 |
| 20x zoom 2 | 0.62 |
| 40x zoom 1 | 0.62 |
| 40x zoom 2 | 0.31 |
| 60x zoom 1 | 0.414 |
| 60x zoom 2 | 0.207 |
Mounted filters
- DAPI ex:377/50 em:447/60
- GFP ex:472/30 em:520/35
- mCherry ex 562/40 em:624/40
- Cy5 ex:628/40 em:692/40
Lasers
- 405nm 100mW
- 488nm 50mW
- 561nm 50mW
- 640nm 40mW
For FRET :
- 440nm 50mW
- 515nm 50mW
For Optogenetic:
- Cool LED 470nm
Computer
- Windows 7 64 bits
- NIS Elements AR V4.30.02 (Build 1053) 64 bit