Nipkow laser photo-manipulations + Temp Controller + CO2 (Tel. 94 787)

Capabilities Confocal_Spinning_Disk.pdf

Fast Imaging living samples
Confocal microscopy (2 pinholes' disks, 25 um in diameter for 20x/40x objectives and 50 um in diameter for 63x/100x objectives)
Widefield Imaging
Temperature (21°C, 37°C), humidity, CO2 control (top stage incubator, OKO lab), autofocus system
Fast dual-colour imaging (2 cameras), ratiometric imaging, FRET technique
Photo-manipulations (-activation, -conversion, -bleaching)
Laser injury

Zeiss Axio Observer 7 (inverted)
CSU W1 automatic Yokogawa spinning disk head (2 disks, 25 and 50 um), 2 camera ports
Definite Focus 3 system

EC Plan-Neofluar 5x / 0.16 Ph1 M27 WD=18.5 mm
EC Plan-Neofluar 10x / 0.3 Ph1 M27 WD=5.2mm
Plan Apochromat 20x / 0.8 Ph2 WD=0.55mm
EC Plan-Neofluar 40x / 1.3 Oil Ph3 M27 Oil immersion WD=0.21mm
LCI Plan-Neoflur 63x / 1.3 Imm Korr DIC for Water Silicone Glycerole immersion WD=0.15-0.17
Alpha Plan Apochromat 100x / 1.46 Oil DIC (UV)VIS-IR WD=0.1mm

pE-800 high-power LED illumination system

Yokogawa dichroic & Microscope filters

-1) D1 Dichroic for multiband 405/488/561/640 nm

-2) D2 Dichroic for multiband 561 LP

Emission-filter camera 01:

* Dapi ET 460/50

* CFP ET 470/24

Emission-filter camera 02:

* GFP ET 525/50

* YFP ET 535/30

* mCherry 605/52

* Cy5 ET 700/75

Lasers for confocal imaging and Photo-manipulations

+ pulsed-laser @UV355 nm

+ 2D Visi-FRAP control 2D scanner

Detectors

PRIME BSI Express back-lluminated cameras

Characteristics:

sCMOS camera x 2

Combined with high sensitive back-illumination

95% Quantum Efficiency

6.5 um x 6.5 um Pixel area

95 Frames per second over USB 3.2 Gen 2

Inserts

35 mm Petric-dish holder (magnetic)

Conventional slide holder (magnetic)

96-well plates holder