Nipkow + Temp Controller + CO2 (Tel. 94 785)
Capabilities Confocal_Spinning_Disk.pdf
- Fast Imaging living samples CFP, YFP and also DAPI, GFP, Rhodamine, Cy5
- Confocal microscopy
- Temperature, humidity, CO2 control
- Peristaltic pump, water bath
- FRET technique
Detailed specifications
Microscope
- Zeiss Cell Observer.Z1 (inverted)
- HAL100 and HXP120C for transmission and fluorescence widefield eyepiece visualization
- CSU X1 automatic Yokogawa spinning disk head
- Dual-View system
- Definite Focus system
Objectives
|
| Objective | Micron/ pixel value |
| 10x | 1.6194 |
| 40x | 0.4073 |
| 63x | 0.2553 |
| 100x | 0.1616 |
Yokogawa Scan Head
1) DAPI 460 / 50
2) Cy5 700 / 75
3) GFP 525 / 50
4) RFP 605 / 70
5) Closed
6) Open
Yokogawa dichroic
1) DAPI-GFP-RFP
2) CFP-YFP-RFP
3) DAPI-GFP-RFP-Cy5
DV2 Split view system
1) CFP - YFP filter T505 / pxr
ET 470 / 24 and ET 535 / 30
2) GFP - Cy3 filter 01-22-EM
ET 525 / 50 and ET 630 / 75
Microscope filters
- FITC
- CFP
- Rhodamine
Lasers
- 405 nm
- 445 nm
- 488 nm
- 561 nm
- 640 nm
Detector
Evolve EM512 camera (Photometrics)
Characteristics:
EMCCD camera
Combined with high sensitive backillumination
512 x 512 Pixel, 16x16 μm , Frame Transfer CCD
16 BIT 16 bit Dual ADC, 10 Mhz, 5MHz and 1,0 Mhz Operation
About 34 full frames/sec
Up to about 748 sub frames/sec (ROI)
Thermoelectrically cooling down to -85°C air cooled about 95% QE
Computer
- Windows 10
- Visiview 4.00.10