Leica TCS SP8 STED 3X (Tel. 94 788)
Capabilities
- Point scanning system
- Super resolution microscopy
- Colocalization, structure/ morphology , 3D- Z-stacks
- Fluorescence Lifetime Imaging Microscopy (FLIM)
- Full living-cell system (LIS system)
Detailed specifications
Microscope
- Inverted Leica DMi8
- Motorized XY-stage
- Lamp of fluorescence: SOLA light engine (LUMENCOR)
- Transmission (diode)
- DFC7000T camera
Objectives
- HC PL Fluotar 5x/ 0.15
- HC PL Fluotar 20x/ 0.55
- HC PL Apo 63x/ 1.40 Oil CS2
- HC PL Apo 93x/ 1.30 GLYCEROL motC STED W
- HC PL Apo 100x/ 1.40 Oil CS2
Conventional mounted filters
- Filter cube DAPI ("Blue")
- Filter cube GFP ("Green")
- Filter cube TxR ("Red")
- Filter cube Y5 ("Far Red")
Lasers
- Pulsed White Laser (80 MHz) fully tunable in the range of 470- 670 nm for maximal spectral flexibility
- UV laser line @405 nm
- Depletion lasers: 592 nm and 775 nm (pulsed laser)
Detectors
- Spectral Detector from 405 nm to 800 nm
- 2 PMT, 2 HyD (including one HyD smd) + transmission PMT
Software
Protocoles
- STED 3X sample preparation (Leica)
- Recommendations: sample preparation (Bioimaging) ****IMPORTANT ****
- Ab: List and references (Bioimaging)
- Expansion STED microscopy: article
- Principle: quick overview
Sample preparation kit provided by the Bioimaging
One kit contains: 100 ul of mounting medium (Prolong Gold), 10 ul of secondary Antibody (Ab), 5ul of PicoGreen (nucleus staining) and the number of coverslips you need.
Secondary antibodies (goat anti-mouse, goat anti-rat, goat anti-rabbit) available are: Abberior STAR 580, Abberior STAR RED.
Download the free lite version from:
(Look at the bottom of the page on the Leica Website for download)
Download the protocole for FRAP experiments: