Axiocam Fluo (Tel. 95 356)

Capabilities

• Multi-channel fluorescence imaging in widefield microscopy
• Apotome 2
• XY motorized stage for large images
• Ideal for thin fixed specimens (oil objectives)

Detailed specifications

Microscope stand

• Zeiss Axio Imager M2 (upright)

Objectives

• EC Plan-Neofluar 5x/0.16 (WD=18.5mm)
• Plan Apochromat  10X/0.45 (WD=2.1mm)
• EC Plan-Apochromat  20X/ 0.8 (WD=0.55mm)
• EC Plan-Apochromat 40x/1.4 Oil (WD=0.13mm)
• Plan Apochromat  63x/1.4 Oil (WD=0.19mm)
• Plan-Apochromat 100x/1.4 Oil (WD=0.17mm)

Sources of illumination

• UV illumination is through a Colibri 5 type RGB-UV (385nm - 475nm - 555nm - 630nm)
• Light illumination is through a 12V halogen lamp 100W, with a condenser 0.9
  Ph1,Ph2,Ph3, with an iris diaphragm

Mounted filter cubes (turret)

• Filter set 96 : DAPI, Hoechst  BP BP390/40, FT 420, BP 450/40
• Filter FITC, GFP :  no exciter, T 495, ET 525/50
• Filter F46-004 : Cy3   ET 545/25, T 565, ET 605/70
• Filter set 50 : Cy5   BP 640/30, FT 660, BP 690/50
• Filter Quad band DAPI-FITC-Cy3-Cy5   Filter-set-Quad-band.jpg

Multichanel acquisitions will be 3 time faster compared to the acquisition made with the single bands

Detector

Axiocam 702 mono (Zeiss)

Characteristics:

Effective pixels : 1920 x 1216, 14 bit/pixel
Electronic shutter (0.1ms to 60 s)

 Computer

• Window 10 64 bits
• Zen 2.6