Axiocam Fluo (Tel. 95 356)
Capabilities
- Multi-channel fluorescence imaging in widefield microscopy
- Apotome 2
- XY motorized stage for large images
- Ideal for thin fixed specimens (oil objectives)

Detailed specifications
Microscope stand
- Zeiss Axio Imager M2 (upright)
Objectives
- EC Plan-Neofluar 5x/0.16 (WD=18.5mm)
- Plan Apochromat 10X/0.45 (WD=2.1mm)
- EC Plan-Apochromat 20X/ 0.8 (WD=0.55mm)
- EC Plan-Apochromat 40x/1.4 Oil (WD=0.13mm)
- Plan Apochromat 63x/1.4 Oil (WD=0.19mm)
- Plan-Apochromat 100x/1.4 Oil (WD=0.17mm)
Objective | Micron/ pixel value |
5x | 1.184 |
10x | 0.592 |
20x | 0.293 |
40x | 0.146 |
63x | 0.093 |
100x | 0.058 |
Sources of illumination
- UV illumination is through a Colibri 5 type RGB-UV (385nm - 475nm - 555nm - 630nm)
- Light illumination is through a 12V halogen lamp 100W, with a condenser 0.9
Ph1,Ph2,Ph3, with an iris diaphragm
Mounted filter cubes (turret)
- Filter set 96 : DAPI, Hoechst BP BP390/40, FT 420, BP 450/40
- Filter FITC, GFP : no exciter, T 495, ET 525/50
- Filter F46-004 : Cy3 ET 545/25, T 565, ET 605/70
- Filter set 50 : Cy5 BP 640/30, FT 660, BP 690/50
- Filter Quad band DAPI-FITC-Cy3-Cy5 Filter-set-Quad-band.jpg
Multichanel acquisitions will be 3 time faster compared to the acquisition made with the single bands
Detector
Axiocam 702 mono (Zeiss)
Characteristics:
Effective pixels : 1920 x 1216, 14 bit/pixel
Electronic shutter (0.1ms to 60 s)
Computer
- Window 10 64 bits
- Zen 2.6