Nikon Widefield P2 (Tel. 94 786)

• BSL-2 environment (NOT for pathogenic bacteria!)
• Please download: BIOIMAGING Standard operating Procedure for BSL2 

Capabilities

• (Fast) Live-cell imaging (environmental chamber, hardware autofocus)
• Multi-colour fluorescence

Microscope

• Nikon ECLIPSE Ti2-E inverted microscope
• OKOlab environmental chamber (temperature and CO2 control)
• Perfect Focus System 3 (PFS-3)
• Several stage inserts (35 mm dish, slides, ...)

Objectives

• CFI PLAN FLUOR DL 4x
• PLAN FLUOR 10X/0.30 PH-1, WD= 16 mm
• CFI PLAN FLUOR DLL 20X
• PL FLUOR 40x/1.30 OIL IMMERSION, WD= 0.24 mm
• PLAN FLUOR 40x/0.75 PH-2, WD= 0.66 mm
• PLAN APO LBDA 60x/1.40 OIL IMMERSION, WD=0.13 mm
• PLAN APO LBDA 100x/1.45 OIL IMMERSION, WD= 0.13 mm

Illumination filter set

-1) Pinkel PENTAchroic for DAPI/ FITC/ TRITC/ CY5/ CY7

2) Pinkel TRIPLEchroic for CFP/ YFP/ mCherry

Type of Illumination

• Lumencor Spectra III (Spectra Light Engine) @ 365/ 440/ 514/ 561/ 594/ 670/ 730 nm

Detector

• Kinetix sCMOS camera (Photometrics): 10 Megapixel, 6.5 um pixel size, 500 frames per second, 29.4 mm field of view, 95% Quantum Efficiency.

Computer

• Windows 7 64bits
• NIS- Elements AR 5.30.06 software