Widefield Scanners (3 systems)

ImageXpress + Temp Controller + CO2 (Tel. 94 782)

Capabilities

  • Automated imaging acquisition (fluorescence in widefield or confocal mode and/or brightfield )
  • Suitable for high-content screening assays and for imaging and analysis of a large sample sets (multi-well plate from 6- to 1536 wells)
  • Ideal for live and fixed specimens
  • Temperature (heating 37°C and cooling 21°C), CO2 control, humidity
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Detailed specifications

Objectives

  •  4X CFI PLAN APO LAMBDA, 0.2 NA, 20mm WD
  • 10x CFI PLAN APO LAMBDA, 0.45 NA, 4 mm WD
  • 20X CFI PLAN APO LAMBDA, 0.75 NA, 1 mm WD
  • 60X VC CFI PLAN APO 1.20 NA WATER IMMERSION, 0.31-0.28mm WD

Additional Objectives

  • 20X, PH1 (0- 2mm) 0.45 NA, 7 mm WD
  • 60X, Super PLAN FLUOR ELWD 0.70 NA, CC 0.1-1.3mm

Sources of Illumination

 (200-250 mW, power output)

  • DAPI : LED 390/22
  • CFP : LED 438/29
  • FITC :LED 475/28
  • YFP : laser à 518 + filtre 510/25
  • Cy3 : LED 555/28
  • Texas Red : LED 576/23
  • Cy5 : laser à 637 + filtre 637/12
  • Cy7 : laser à 748 + filtre 748/12

Filters in 5-position Dichroic Wheel

  • Position 1 Multi-Band Dapi, FITC, Cy3, Cy5 and Cy7 (FLd-415-500-581-652-757)
  • Position 2 Dichroic for Texas Red (Di02-R594)
  • Position 3 Multi-Band for CFP and YFP (ZT-445-520-rpc-UF1)
  • Position 4 Empty
  • Position 5 Empty

Filters in 8- position Emission Filter Wheel

  • Position 1 Single-Band Emitter for DAPI  432/36
  • Position 2 Single-Band Emitter for FITC  520/35
  • Position 3 Single-Band Emitter for Cy3  600/37
  • Position 4 Single-Band Emitter for CY5  692/40
  • Position 5 Single-Band Emitter for CY7  819/44
  • Position 6 Single-Band Emitter for CFP  482/25
  • Position 7 Single-Band Emitter for YFP  562/40
  • Position 8 Single-Band Emitter for Texas Red  631/36

Spinning Disk Confocal

Dual disk unit allowing selection between 3 modes A) 60 micron pinhole disk for your basic confocal requirements B) 50 micron slit for high throughput confocal requirements and C) wide field (not-confocal) imaging mode.

Detector

Andor Zyla 4.2 Camera , sCMOS, 4.2 MegaPixels, 100 fps, 16 Bytes, 82% max. QE.

Computer

  • Window 10
  • MetaXpress Version 6.6.1.42