FRET in HeLa cells (FRET pair: CFP/YFP)

This sequence was acquired with the ImageXpress. This video shows that is possible to perform this type of experiments at the Bioimaging core facility. In term of biology, these cells were -unfortunatly- probably dying: the FRET ratio was dramatically perturbed by the loss of focus ... (Image source: ©G. Jacquemin, Martinvalet group, PHYM dpt, CMU)