Confocal Microscopy (4 systems)

Nikon A1r + Temp Controller + CO2 (Tel. 94 783)

Capabilities     Confocal_NikonA1r.pdf

  • Imaging live samples- any fluorescent proteins
  • Temperature, humidity, CO2 control
  • Peristaltic pump, water bath
  • Colocalization, F-techniques (FRAP, FRET, ...)
  • Multiposition time-lapse
  • Tiled images
  • Optogenetic

Nikon A1r web full.jpg

Detailed specifications

Microscope

  • Nikon A1r microscope (inverted)
  • Resonant scanner for fast imaging up to 30 fps
  • DIC prisme
  • Motorised XY stage
  • Perfect focus sytem (PFS):

PSF detail.png

 

Objectives

  • 10x 0.45 CFI Plan Apochromat WD:4mm  (MRD00101)
  • 20x 0.45 CFI ELWD Plan Fluor WD:8.2-6.9mm correction:0-2mm  (MRH08220)
  • 20x 0.75 CFI Plan Apochromat VC WD:1mm  (MRD70200)
  • 40x 0.6 CFI ELWD S Plan Fluor WD:3.6-2.8mm correction 0-2mm  (MRH08430)
  • 40x 1.3 CFI Plan Fluor WD:0.2mm  (MRH01401)
  • 60x 1.4 CFI Plan Apochromat VC WD:0.13mm  (MRD01602)

And also into the cupboard : 100x 1.4 CFI Plan Apochromat VC WD:0.13mm  (MRD01901)

Objective Micron/ pixel value
20x zoom 1 1.24
20x zoom 2 0.62
40x zoom 1 0.62
40x zoom 2 0.31
60x zoom 1 0.414
60x zoom 2 0.207

 

Mounted filters

  • DAPI ex:377/50 em:447/60
  • GFP ex:472/30 em:520/35
  • mCherry ex 562/40 em:624/40
  • Cy5 ex:628/40 em:692/40

Lasers

  • 405nm  100mW
  • 488nm  50mW
  • 561nm  50mW
  • 640nm  40mW

For FRET :

  • 440nm  50mW
  • 515nm  50mW

For Optogenetic:

  • CoolLED pE-100 at 470nm

Computer

  • Windows 7 64 bits
  • NIS Elements AR V4.30.02 (Build 1053) 64 bit