18 octobre 2018: prof. John Yates
Ernest W. Hahn Professor
Departments of Molecular Medicine and Neurobiology
The Scripps Research Institute - LaJolla, California
«Understanding the Molecular Defect in Cystic Fibrosis»
A component to understanding biological processes involves identifying the proteins expressed in cells as well as their modifications and the dynamics of processes. Several major technologies, but especially mass spectrometry, have benefited from large-scale genome sequencing of organisms. The sequence data produced by these efforts can be used to interpret mass spectrometry data of proteins and thus enables rapid and large-scale analysis of protein data from experiments. Advances in multi-dimensional separations as well as mass spectrometry have improved the scale of experiments for protein identification. This has improved the analysis of protein complexes, and more complicated protein mixtures. Quantitative mass spectrometry can be used to study biological processes such as protein-protein interactions, development or the effects of gene mutations on pathways. Recent studies on the loss of function mutant form of the Cystic Fibrosis Transport Regulator (DF508) as it progresses through the folding pathway will be presented. Through the study of protein-protein interactions and modifications that regulate maturation of CFTR, we now have a better understanding of the underlying biochemical process leading to loss of function.
Pankow S, Bamberger C, Calzolari D1, Martínez-Bartolomé S, Lavallée-Adam M2, Yates J
1Qualcomm, San Diego, CA
2Department of Biochemistry, Microbiology and Immunology, Ottawa Institute of Systems Biology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario. KlH 8M5
1Pankow et al Nature 2015, 528, 510-6., Pankow et al Science Signaling (in press)
This work was supported by National Institutes of Health grants 5R01HL079442-08 (to J.R.Y. and W.E.B.), P01AG031097 (to J.R.Y.), P41 GM103533 (to J.R.Y.), HHSN268201000035C (to J.R.Y.), and a Cystic Fibrosis Foundation mass spectrometry fellowship BALCH050X6 (to S.P. and J.R.Y.). M.L.-A. holds a postdoctoral fellowship from Fonds de recherche du Québec - Nature et technologies
John R. Yates is the Ernest W. Hahn Professor in the Departments of Molecular Medicine and Neurobiology at The Scripps Research Institute. His research interests include development of integrated methods for tandem mass spectrometry analysis of protein mixtures, bioinformatics using mass spectrometry data, and biological studies involving proteomics. He is the lead inventor of the SEQUEST software for correlating tandem mass spectrometry data to sequences in the database and developer of the shotgun proteomics technique for the analysis of protein mixtures. His laboratory has developed the use of proteomic techniques to analyze protein complexes, posttranslational modifications, organelles and quantitative analysis of protein expression for the discovery of new biology. Many proteomic approaches developed by Yates have become a national and international resource to many investigators in the scientific community. He has received the American Society for Mass Spectrometry research award, the Pehr Edman Award in Protein Chemistry, the American Society for Mass Spectrometry Biemann Medal, the HUPO Distinguished Achievement Award in Proteomics, Herbert Sober Award from the ASBMB, and the Christian Anfinsen Award from The Protein Society, the 2015 ACS’s Analytical Chemistry award, 2015 The Ralph N. Adams Award in Bioanalytical Chemistry and the 2018 Thomson Medal from the International Mass Spectrometry Society. He was ranked by Citation Impact, Science Watch as one of the Top 100 Chemists for the decade, 2000-2010. He was #1 on a List of Most Influential in Analytical Chemistry compiled by The Analytical Scientist 10/30/2013 and is on the List Of Most Highly Influential Biomedical Researchers, 1996-2011, European J. Clinical Investigation 2013, 43, 1339-1365 and the Thomson Reuters 2015 List of Highly Cited Scientists. He has published over 900 scientific articles with ~118,000 citations, and an H index greater than 172 (Google Scholar). Dr. Yates is the Editor in Chief at the Journal of Proteome Research.
Frontiers in biomedicine