Reactive oxygen species (ROS)
NADPH oxidases in the central nervous system: NOX enzymes are found in different part of the central nervous system with different functions. We are presently working on the role of the phagocyte NADPH oxidase NOX2 and reactive oxygen species (ROS) in neurogenic regions of the adult CNS. ROS at physiological levels are involved in neuroregeneration, while excessive amounts of ROS may lead to neurodegeneration. At present are particularly intrigued by the phenomenon of promoter switch, which allows NOX2 expression in the brain.
NADPH oxidases in the inner ear: NOX3 is the predominant NADPH oxidase of the inner ear, where it is involved in basic functions (in particular otolith formation), however when over expressed (due to noise, drugs, or age), it contributes to hearing loss. At present we are developing NOX3 targeted otoprotective therapies
Therapeutic minigenes to allow vector-mediated gene knock-down: This technology has been developed in our lab over the last decade and is not applied to develop innovative molecular therapies, for the inner, for HIV infection, and in particular for immune exhaustion.
Measurements of reactive oxygen species
- Vector-mediated gene knock-down
Rousset F, Nacher-Soler G, Coelho M, Ilmjarv S, Kokje VBC, Marteyn A, Cambet Y, Perny M, Roccio M, Jaquet V, Senn P, Krause KH. Redox activation of excitatory pathways in auditory neurons as mechanism of age-related hearing loss. Redox Biol. 2020 Feb;30:101434. doi: 0.1016/j.redox.2020.101434. Epub 2020 Jan 20. PMID: 32000019; PMCID: PMC7016250
Rousset F, Salmon P, Bredl S, Cherpin O, Coelho M, Myburgh R, Alessandrini M, Perny M, Roccio M, Speck RF, Senn P, Krause KH. Optimizing Synthetic miRNA Minigene Architecture for Efficient miRNA Hairpin Concatenation and Multi-target Gene Knockdown. Mol Ther Nucleic Acids. 2018 Dec 14;14:351-363
Seredenina T, Sorce S, Herrmann FR, Ma Mulone XJ, Plastre O, Aguzzi A, Jaquet V, Krause KH. Decreased NOX2 expression in the brain of patients with bipolar disorder: association with valproic acid prescription and substance abuse. Transl Psychiatry. 2017 Aug 15;7(8):e1206
Nayernia Z, Colaianna M, Robledinos-Antón N, Gutzwiller E, Sloan-Béna F, Stathaki E, Hibaoui Y, Cuadrado A, Hescheler J, Stasia MJ, Saric T, Jaquet V, Krause KH. Decreased neural precursor cell pool in NADPH oxidase 2-deficiency: From mouse brain to neural differentiation of patient derived iPSC. Redox Biol. 2017 Apr 24;13:82-93
Cosset E, Martinez Y, Preynat-Seauve O, Lobrinus JA, Tapparel C, Cordey S, Peterson H, Petty TJ, Colaianna M, Tieng V, Tirefort D, Dinnyes D, Dubois-Dauphin M, Kaiser L, Karl-Heinz Krause: Human three-dimensional engineered neural tissue reveals cellular and molecular events following cytomegalovirus infection, Biomaterials, June 2015, Volume 53:296-308
Host response to pathogens