BioCode:R2P expertise

Services

Ribosome fractionation

One of the most critical factors for optimal ribosome purification is the quality of the cells from which the ribosomes are to be isolated. The best material is usually obtained from cells growing rapidly in a rich medium at their optimal temperature and harvested in early to mid-log phase long before growth and translation start to slow. For high yields of polysomes, preserve the polysomes by chilling the culture rapidly and adding of chloramphenicol (for prokaryotes) or cycloheximide (for eukaryotes) before harvest are required. Best conditions for polysome preparations vary from different organisms and tissues.

  • Cell culturing and sample preparation for ribosome fractionation
  • Ribosome fractionation in sucrose gradient

RNA isolation for RNA-Seq

  • Cell culturing
  • RNA isolation for libraries with QC

Analysis of translatome by Ribosome profiling (Ribo-Seq)

  • Conditions for optimal polysome preparation
  • Ribo-Seq library preparation with rRNA depletion

cell/tissue disruption by cryo-mill in n2

  • Suitable for yeast, Gram+ bacteria, plants, hard tissues

Analysis of protein solubility

  • Cell culturing and sample preparation
  • Isolation of aggregated proteins

In vitro translation in cell-free protein systems (CFPS)

  • Cell culturing
  • Preparation of CFPS from customer cells
  • In vitro transcription of mRNA transcripts, capping, polyA tailing
  • Analysis of the protein production by SDS-PAGE/Western blot/luminescence