EQUIPMENT

READERS

Multimode Readers: for 24, 48,96 &384 well Plates, either in endpoint or Kinetics measurement

 1. FLEXSTATION 3 from Molecular Devices

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 Readouts: absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence.

 Liquid transfer station included

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2. SPECTRAMAX PARADIGM from Molecular Devices

Readouts: absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence.

PARADIGM.png                  CARTRIDGE.jpg           TUNE CARTRIDGE.jpg

         

3. FLUOSTAR OPTIMA from BMG

 FLUOSTAR.jpg

Readouts: absorbance, fluorescence intensity, fluorescence polarization, luminescence and time-resolved fluorescence.

Equipped with a injector

 

2. ENSPIRE  from PERKINELMER

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Readouts : absorbance, luminescence, ALPHALISA Technology

Luminescence Reader

SpectraMax L 96w from Molecular devices

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Reader equipped with 2 injectors which is dedicated for Luminescence  for 96w plate

SpectraMax L 384w from Molecular devices

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Reader equipped with 2 injectors which is dedicated for Luminescence for 96 & 384w plate

Equipped with BRET 1 & BRET 2 Option

XFe96 ANALYSER SEAHORSE BIOSCIENCE

SEAHORSE XF96 ANALYSER

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The Seahorse XFe96 Analyzer measures OCR and ECAR of live cells in a 96-well plate format.
These rates are key indicators of mitochondrial respiration and glycolysis and provide
a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples

 

        Web Site
       User_Guide.pdf

PROTOCOLS

  1. XFe96_PLANNING_MATERIALS.pdf
  2. XFe96_PLANNING_GLYCO_KIT_PREPARATION.pdf
  3. XFE96_DAY_OF_RUNNING_ASSAY_CELL_DENSITY_AND_OLIGO_OPT.pdf
  4. XFe96_DAY_OF_WASHING_CELLS.pdf
  5. XFe96_DAY_BEFORE_CARTRIDGE_HYDRATION.pdf
  6. XFe96_DAY_BEFORE_CELL_SEEDING.pdf
  7. XFe96_DAY_OF_COMPOUNDS_FOR_GLYCO_ASSAY.pdf
  8. XFe96_DAY_OF_MEDIA_PREP.pdf
  9. XFe96_DAY_OF_LOADING_CARTRIDGE.pdf