Prices
| Deliverable | Price (CHF) |
Details
|
| Standard cloning | 500 |
Your gene of interest is inserted in a pENTR plasmid by RE cloning
|
| Gateway cloning | 400 |
Your gene of interest in inserted in a pENTR plasmid by PCR and Gateway cloning (BP reaction)
|
| microRNA cloning | 800 |
Your microRNA is designed by us, ordered for gene synthesis with 3 hairpins, then cloned in a pENTR plasmid by RE cloning
|
| Final cloning | 400 |
Your pENTR plasmid is cloned in a lentivector plasmid (monocistronic, bicistronic, autoinducible) by Gateway cloning (LR reaction). The final plasmid is maxipreped (average yield is 500 µg)
|
| Lentivector production | 350 |
Your lentivector is produced, concentrated and aliquoted. Average yield. Average concentration.
|
| Lentivector titration by FACS | 150 |
Your lentivector is titrated on HT-1080 cells and by FACS (must contain a living color and a promoter active in HT-1080 cells)
|
| Lentivector titration by qPCR | 200 | In all other situations, your lentivector is titrated on HT-1080 cells and by qPCR. |