Lentivector titration by FACS

This method can only be used to titer stocks of vectors that carry a transgene that is easily monitored by FACS (such as GFP, or any living colors, or any membrane protein that can be detected by flow cytometry), and whose expression is governed by a promoter that is active in Hela cells (tissue-specific promoter-containing vector must be functionally assayed in specific cells, and titered by QPCR in Hela cells.

For any other situation, we need to perform a qPCR assay on genomic DNA of transduced cells. For further details on lentivector titration, you can check one of our recent bookchapter, by clicking on the picture below.