Publications

Google scholar

 

Peer-reviewed publications in international scientific journals (independent career)

 

56. “Following Electroenzymatic Hydrogen Production by Rotating Ring Disk Electrochemistry and Mass Spectrometry”

Khushvakov, J., Nussbaum, R., Cadoux, C., Duan, J., Stripp, S.T. and Milton, R.D.* (2021).

Angewandte Chemie International Edition60(18), 10001-10006.

 DOI: 10.1002/anie.202100863

  

55. “Natural and Engineered Electron Transfer of Nitrogenase”

Gu, W. and Milton, R.D.* (2020).

Chemistry, 2(2), 322-346.

DOI: 10.3390/chemistry2020021

** Invited Contribution **

  

54. “Recent Enzymatic Electrochemistry for Reductive Reactions”

Cadoux, C. and Milton, R.D.* (2020).

ChemElectroChem7(9):1974-1986

DOI: 10.1002/celc.202000282

** Invited Contribution **

 

53. “Nitrogenase Bioelectrochemistry for Electrosynthesis Applications”

 Milton, R.D.* and Minteer, S.D.* (2019).

 Accounts of Chemical Research, 51(12):3351-3360

DOI: 10.1021/acs.accounts.9b00494

** Invited Contribution **

 

 

 

Peer-reviewed publications in international scientific journals (during PostDoc/PhD studies)

52. Enhanced Electrosynthetic Hydrogen Evolution by Hydrogenases Embedded in a Redox-Active Hydrogel

Ruth, J.C., Milton, R.D., Gu, W. and Spormann, A.M.* (2020).

Chemistry - A European Journal, 26(32):7323-7329

DOI: 10.1002/chem.202000750

   

51. Pyrene-based Noncovalent Immobilization of Nitrogenase on Carbon Surfaces

 Patel, J., Cai, R., Milton, R.D., Chen, H. and Minteer, S.D.* (2019).

 ChemBioChem, 21(12):1729-1732

DOI: 10.1002/cbic.201900697

  

50. Microbial Battery Powered Enzymatic Electrosynthesis for Carbon Capture and Generation of Hydrogen and Formate from Dilute Organics

Dubrawski, K.L., Shao, X., Milton, R.D., Deutzmann, J.S., Spormann, A.M.* and Criddle, C.S.* (2019).

ACS Energy Letters, 4(12):2929-2936

DOI: 10.1021/acsenergylett.9b02203

 

49. “Efficient NADH Regeneration by a Redox Polymer-Immobilized Enzymatic System”

Yuan, M., Kummer, M., Milton, R.D., Quah, T. and Minteer, S.D.* (2019).

ACS Catalysis, 9(6):5486-5495

 

48. “Following Nature: Bioinspired Mediation Strategy for Gram-Positive Bacterial Cells”

Pankratova, G., Pankratov, D., Milton, R.D., Minteer, S.D. and Gorton, L.* (2019).

Advanced Energy Materials, 9(16):1900215

 

47. “Performance Comparison of Different Configurations of Glucose/O2 Microfluidic Biofuel CellStack”

Escalona-Villalpando, R.A., Hasan, K., Milton, R.D., Morena-Zuria, A., Arriaga, L.G., Minteer, S.D. and Ledesma-Garcia, J.* (2019).

Journal of Power Sources, 414:150-157

 

46. Methanococcus maripaludis Employs Three Functional Heterodisulfide Reductase Complexes forFlavin-Based Electron Bifurcation Using Hydrogen and Formate”

Milton, R.D., Ruth, J.C., Deutzmann, J.S. and Spormann, A.M.* (2018).

Biochemistry, 57(32):4848-4857

 

45. “Improved Performance of a Paper-Based Glucose Fuel Cell by Capillary Induced Flow”

Del Torno-de Roman, L., Navarro, M., Hughes, G., Esquivel, J.P., Milton, R.D., Minteer, S.D. and Sabate, N.* (2018).

Electrochimica Acta, 282:336-342

 

44. “Catalysts for Nitrogen Reduction to Ammonia”

Foster, S.L., Perez Bakovic, S.I., Duda, R., Maheshwari, S., Milton, R.D., Minteer, S.D.*, Janik, M.J.*, Renner, J.N.* and Greenlee, L.F.* (2018).

Nature Catalysis, 1:490-500

 

43. “Creating a Low-Potential Redox Polymer for Efficient Electroenzymatic CO2 Reduction”

Yuan, M., Sahin, S., Cai, R., Abdellaoui, S., Hickey, D.P., Minteer, S.D. and Milton, R.D.* (2018).

Angewandte Chemie International Edition, 57(22):6582-6586

 

42. “Electroenzymatic C-C Bond Formation from CO2

Cai, R., Milton, R.D., Abdellaoui, S., Park, T., Patel, J., Alkotaini, B. and Minteer, S.D.* (2018).

Journal of the American Chemical Society, 140(15):5041-5044

 

41. “Molybdenum-Dependent Formate Dehydrogenase for Formate Bioelectrocatalysis in a Formate/O2Enzymatic Fuel Cell”

Sahin, S., Cai, R., Milton, R.D., Abdellaoui, S., Macazo, F.C. and Minteer, S.D.* (2018).

Journal of the Electrochemical Society, 165(3):H109-H113

 

40. “Mediator-free enzymatic electrosynthesis of formate by the Methanococcus maripaludis heterodisulfide reductase supercomplex”

Lienemann, M., Deutzmann, J.S., Milton, R.D., Sahin, M. and Spormann, A.M.* (2018).

Bioresource Technology, 254:278-283

  

39. “Mechanism of nitrogenase H2 formation by metal-hydride protonation probed by mediatedelectrocatalysis and H/D isotope effects”

Khadka, N., Milton, R.D., Shaw, S., Lukoyanov, D., Dean, D., Minteer, S.D., Raugei, S., Hoffman, B.M.* and Seefeldt, L.C.* (2017).

Journal of the American Chemical Society, 139(38):13518-13524

 

38. “Hybrid bioelectrocatalytic reduction of oxygen at anthracene-modified multi-walled carbonnanotubes decorated with Ni90Pd10 nanoparticles”

Aquino Neto, S., Da Silva, R.G., Milton, R.D., Minteer, S.D.* and De Andrade, A.R.* (2017).

Electrochimica Acta, 251:195-202

 

37. “Enhanced bioelectrocatalysis of Shewanella oneidensis MR-1 by a naphthoquinone redox polymer”

Hasan, K., Grattieri, M., Wang, T., Milton, R.D., and Minteer, S.D.* (2017).

ACS Energy Letters, 1:1568-1572

 

36. “Investigating extracellular electron transfer of Rikenella microfusus: A recurring bacterium in mixed-species biofilms”

Grattieri, M., Hasan, K., Milton, R.D., Abdellaoui, S., Suvira, M., Alkotaini, B. and Minteer, S.D.* (2017).

Sustainable Energy Fuels, 1:1568-1572

 

35. “Direct enzymatic bioelectrocatalysis: Differentiating between myth and reality”

Milton, R.D. and Minteer, S.D.* (2017).

Journal of the Royal Society: Interface, 14(131):20170253

** Invited Contribution **

 

34. “Bioelectrocatalytic NAD+/NADH Inter-Conversion: Transformation of an Enzymatic Fuel Cell into an Enzymatic Redox Flow Battery”

Quah, T., Milton, R.D., Abdellaoui, S. and Minteer, S.D.* (2017).

Chemical Communications, 53:8411-8414

 

33. “The In Vivo Potential-Regulated Protective Protein of Nitrogenase in Azotobacter vinelandii Supports Aerobic Bioelectrochemical Dinitrogen Reduction In Vitro”

Milton, R.D., Cai, R., Sahin, S., Abdellaoui, S., Alkotaini, B., Leech, D. and Minteer, S.D.* (2017).

Journal of the American Chemical Society, 139(26):9044-9052

 

32. “Photobioelectrocatalysis of Intact Chloroplasts for Solar Energy Conversion”

Hasan, K., Milton, R.D., Grattieri, M., Wang, T., Stephanz, M. and Minteer, S.D.* (2017).

ACS Catalysis, 7(4):2257-2265

 

31. “Substrate Channeling in an Artificial Metabolon: A Molecular Dynamics Blueprint for an Experimental Peptide Bridge”

Liu, Y., Hickey, D.P., Guo, J-Y., Earl, E., Abdellaoui, S., Milton, R.D., Sigman, M.S.,* Minteer, S.D.* and Calabrese-Barton, S.* (2017).

ACS Catalysis, 7(4):2486-2493

 

30. “Bioelectrochemical Haber-Bosch process: An Ammonia-Producing H2/N2 Fuel Cell”

Milton, R.D., Cai, R., Abdellaoui, S., Leech, D., De Lacey, A.L., Pita, M. and Minteer, S.D.* (2017).

Angewandte Chemie International Edition, 56(10):2680-2683

** Deemed “Very Important Paper” by reviewers **

** Press release **

  

29. “Rechargeable Membraneless Glucose Biobattery: Towards Solid-State Cathodes for Implantable Enzymatic Devices”

Ahmadian-Yazdi, A., Preite, R., Milton, R.D., Hickey, D.P., Minteer, S.D. and Xu, J.* (2017).

Journal of Power Sources, 343:103-108

 

28. “Improving the Performance of Lactate/Oxygen Biofuel Cells Using a Microfluidic Design”

Escalona-Villalpando, R.A., Reid, R.C., Milton, R.D., Arriaga, L.G., Minteer, S.D. and Ledesma-Garcia, J.* (2017).

Journal of Power Sources, 342:546-552

 

27. “Rational Combination of Promiscuous Enzymes Yields a Versatile Enzymatic Fuel Cell with Improved Coulombic Efficiency”

Holade, Y., Yuan, M., Milton, R.D., Hickey, D.P., Sugawara, A., Peterbauer, C.K., Haltrich, D. and Minteer, S.D.* (2017).

Journal of the Electrochemical Society, 164(3):H3073-H3082

 

26. “Cholesterol as a Promising Alternative Energy Source: Bioelectrocatalytic Oxidation Using NAD-Dependent Cholesterol Dehydrogenase in Human Serum”

Quah, T., Abdellaoui, S., Milton, R.D., Hickey, D.P. and Minteer, S.D.* (2017).

Journal of the Electrochemical Society, 164(3):H3024-H3029

 

25. “Enzymatic Bioelectrosynthetic Ammonia Production: Recent Electrochemistry of Nitrogenase, Nitrate Reductase, and Nitrite Reductase”

Milton, R.D.* and Minteer, S.D. (2016).

ChemPlusChem, 82(4):513-521

 

24. “Improving O2 reduction at an enzymatic biocathode: mimicking the lungs”

Hickey, D.P., Knoche, K.L., Albertson, K., Castro, C., Milton, R.D. and Minteer, S.D.* (2016).

Chemical Communications, 52:13299-13302

 

23. “Wiring of Photosystem I and Hydrogenase on an Electrode for Photoelectrochemical H2 Production by using Redox Polymers for Relatively Positive Onset Potential”

Tapia, C.,* Milton, R.D., Pankratova, G., Minteer, S.D., Åkerlund, H.E., Leech, D., De Lacey, A.L., Pita, M.* and Gorton, L. (2016).

ChemElectroChem, (4)1:90-95

 

22. “Hybrid Glucose/O2 Biobattery and Supercapacitor Utilizing a Pseudocapacitive Dimethylferrocene Redox Polymer at the Bioanode”

Knoche, K.L., Hickey, D.P., Milton, R.D., Curchoe, C.L. and Minteer, S.D.* (2016).

ACS Energy Letters, 1(2):380-385.

** Featured in Chemical & Engineering News **

 

21. “Nitrogenase bioelectrocatalysis: heterogeneous ammonia and hydrogen production by MoFe protein”

Milton, R.D., Abdellaoui, S., Khadka, N., Dean, D.R., Leech, D., Seefeldt, L. and Minteer, S.D.* (2016).

Energy & Environmental Science, 9:2550-2554.

 

20. “Membraneless enzymatic ethanol/O2 fuel cell: Transitioning from an air-breathing Pt-based cathode to a bilirubin oxidase-based biocathode”

Aquino Neto, S., Milton, R.D., Hickey, D.P., De Andrade, A.R. and Minteer, S.D.* (2016).

Journal of Power Sources, 324:208-214.

  

19. “Laccase Inhibition by Arsenite/Arsenate: Determination of Inhibition Mechanism and Preliminary Application to a Self-Powered Biosensor”

Wang, T., Milton, R.D., Abdellaoui, S., Hickey, D.P. and Minteer, S.D.* (2016).

Analytical Chemistry, 88(6):3243-3248.

 

18. “Tailoring Biointerfaces for Electrocatalysis”

Milton, R.D., Wang, T., Knoche, K.L. and Minteer, S.D.* (2016).

Langmuir, 32(10):2291-2301.

** Invited Feature Article (Front Cover) **

 

17. “NAD-dependent dehydrogenase bioelectrocatalysis: the ability of a naphthoquinone redox polymer to regenerate NAD”

Abdellaoui, S., Milton, R.D., Quah, T. and Minteer, S.D.* (2016).

Chemical Communications, 52:1147-1150.

 

16. “A self-powered amperometric lactate biosensor based on lactate oxidase immobilized in dimethylferrocene-modified LPEI”

Hickey, D.P., Reid, R.C., Milton, R.D. and Minteer, S.D.* (2016).

Biosensors & Bioelectronics, 77:26-31.

 

15. “Bioelectrochemical Study of Thermostable Pycnoporus sanguineus CS43 Laccase Bioelectrodes Based on Pyrolytic Carbon Nanofibers for Bioelectrocatalytic O2 Reduction”

Holmberg, S., Rodriguez-Delgado, M., Milton, R.D., Ornelas-Soto, N., Minteer, S.D., Parra, R. and Madou, M.J.* (2015).

ACS Catalysis, 5:7507-7518.

 

14. “Promiscuous Glucose Oxidase: Electrical Energy Conversion of Multiple Polysaccharides Spanning Starch and Dairy Milk”

Milton, R.D., Wu, F., Lim, K., Abdellaoui, S., Hickey, D.P. and Minteer, S.D.* (2015).

ACS Catalysis, 5:7218-7225.

 

13. “TEMPO-Modified Linear Poly(ethylenimine) for Immobilization-Enhanced Electrocatalytic Oxidation of Alcohols”

Hickey, D.P., Milton, R.D., Chen, D., Sigman, M.S. and Minteer, S.D.* (2015).

ACS Catalysis, 5:5519-5524.

 

12. “Rational design of quinones for high power density biofuel cells”

Milton, R.D., Hickey, D.P., Abdellaoui, S., Lim, K., Wu, F., Tan, B. and Minteer S.D.* (2015).

Chemical Science, 6:4867-4875.

 

11. “High current density PQQ-dependent alcohol and aldehyde dehydrogenase bioanodes”

Aquino Neto, S., Hickey, D.P., Milton, R.D., De Andrade, A.R. and Minteer, S.D.* (2015).

Biosensors & Bioelectronics, 72:247-254.

 

10. “Employing FAD-dependent glucose dehydrogenase within a glucose/oxygen enzymatic fuel cell operating in human serum”

Milton, R.D., Lim, K, Hickey, D.P. and Minteer, S.D.* (2015).

Bioelectrochemistry, 106 Part A:56-63.

 

9. “Co-immobilization of gold nanoparticles with glucose oxidase to improve bioelectrocatalytic glucose oxidation

Aquino Neto, S., Milton, R.D., Crepaldi, L.B., Hickey, D.P., De Andrade, A.R. and Minteer, S.D.* (2015).

Journal of Power Sources, 285:493-498.

 

8. “Regeneration of the NADH Cofactor by a Rhodium Complex Immobilized on Multi-Walled Carbon Nanotubes”

Tan, B., Hickey, D.P., Milton, R.D., Giroud, F. and Minteer, S.D.* (2015).

Journal of the Electrochemical Society, 162(3):H102-107.

 

7. “Simplifying Enzymatic Biofuel Cells: Immobilized Naphthoquinone as a Biocathodic Orientational Moiety and Bioanodic Electron Mediator”

Giroud, F., Milton, R.D., Tan, B. and Minteer, S.D.* (2015).

ACS Catalysis, 5:1240-1244.

 

6. “Investigating the Reversible Inhibition Model of Laccase by Hydrogen Peroxide for Bioelectrocatalytic Applications”

Milton, R.D. and Minteer, S.D.* (2014).

Journal of the Electrochemical Society, 161:H3011-3014.

 

5. “From PEM Fuel Cell Design to Biological Fuel Cells: The Status of Systems Development for Biological Fuel Cells

Rasmussen, M. Milton, R.D., Hickey, D.P., Reid, R.C. and Minteer, S.D.* (2014).

Electrochemical Society (ECS) Transactions, 64(3):881-895.

** Invited Review Article **

 

4. “Glucose oxidase progressively lowers bilirubin oxidase bioelectrocatalytic cathode performance in single-compartment glucose/oxygen biological fuel cells”

Milton, R.D., Giroud, F., Thumser, A.E., Minteer, S.D. and Slade, R.C.T.* (2014).

Electrochimica Acta, 140:59-64.

 

3. “Bilirubin oxidase bioelectrocatalytic cathodes: the impact of hydrogen peroxide”

Milton, R.D., Giroud, F., Thumser, A.E., Minteer, S.D. and Slade, R.C.T.* (2014).

Chemical Communications, 50 (1):94-96.

 

2. “Hydrogen peroxide produced by glucose oxidase affects the performance of laccase cathodes in glucose/oxygen fuel cells: FAD-dependent glucose dehydrogenase as a replacement”

Milton, R.D., Giroud, F., Thumser, A.E., Minteer, S.D. and Slade, R.C.T.* (2013).

Physical Chemistry Chemical Physics, 15 (44):19371-19379.

 

1. “An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer”

Milton, R.D., Baur, J., Varcoe, J.R., Thumser, A.E. and Slade, R.C.T.* (2012).

Physical Chemistry Chemical Physics, 14 (27):9582-9585.

  

 

Contributions to books

2. “FAD-Dependent Glucose Dehydrogenase Immobilization and Mediation Within a Naphthoquinone Redox Polymer”

Milton, R.D. in Enzyme Stabilization and Immobilization: 2nd Ed. (2016).

Springer, DOI: 10.1007/978-1-4939-6499-4_15

 

1. “Fundamentals and applications of bioelectrocatalysis”

Hickey, D.P., Milton, R.D., Rasmussen, M., Abdellaoui, S., Nguyen, K. and Minteer, S.D. in Electrochemistry: Volume 13 (2016).

Royal Society of Chemistry, vol. 13, pp. 97-132.

  

 

Oral contributions to international conferences(as the presenting author)

9. “Flavin-based electron bifurcation in Methanococcus maripaludis”

Milton, R.D., Ruth, J.C., Gu, W., Deutzmann, J.S., Shima, S. and Spormann, A.M.

Presented at the Electrochemical Conference on Energy and the Environment 2019: Bioelectrochemistry and Energy Storage (ECS Satellite Conference) – Glasgow, Scotland July 2019.

    

8. “Flavin-based electron bifurcating enzyme complexes in Methanococcus maripaludis”

Milton, R.D., Ruth, J.C., Deutzmann, J.S., Shima, S. and Spormann, A.M.

Presented at the 2017 meeting of West Coast Bacterial Physiologists – Asilomar, CA, USA, December 2017.

 

7. “Bioelectrochemical Haber-Bosch process: Coupling hydrogenase and nitrogenase to create an ammonia-producing H2/N2 fuel cell”

Milton, R.D., Cai, R., Abdellaoui, S., Leech, D., De Lacey, A.L., Pita, M. and Minteer, S.D.

Presented at the 68th Annual Meeting of the International Society of Electrochemistry – Providence, RI, USA, September 2017.

** Received Travel Award **

 

6. “Heterogeneous bioelectrochemistry of nitrogenase”

Milton, R.D., Abdellaoui, S., Leech, D. and Minteer, S.D.

Presented at the 67th Annual Meeting of the International Society of Electrochemistry – Netherlands, August 2016.

 

5. “Deep Oxidation of Multiple (Poly)Saccharides at a Bi-Enzymatic Bioelectrode”

Milton, R.D., Yuan, M., Hickey, D.P., Sugawara, A., Peterbauer, C.K., Haltrich, D. and Minteer, S.D.

Presented at the 229th Electrochemical Society meeting, San Diego (USA) – May 2016.

 

4. “The application and immobilization of a novel naphthoquinone derivative for mediated bioelectrocatalysis of glucose by FAD-dependent glucose dehydrogenase”

Milton, R.D., Hickey, D.P., Abdellaoui, S., Lim, K., Tan, B. and Minteer, S.D.

Presented at the 2015 Pacifichem meeting, Honolulu (USA) – December 2015.

 

3. “Naphthoquinone derivatives as low-potential electron mediators of FAD-dependent glucose dehydrogenase”

Milton, R.D., Hickey, D.P., Abdellaoui, S., Lim, K., Tan, B., Wu, F. and Minteer, S.D.

Presented at the 66th Annual Meeting of the International Society of Electrochemistry, Taiwan, October 2015.

 

2. “Engineering glucose oxidase to exhibit enhanced substrate promiscuity for biofuel cell applications”

Milton, R.D., Wu, F., Lim, K., Abdellaoui, S., Hickey, D.P. and Minteer, S.D.

Presented at the biannual meeting of the Bioelectrochemical Society, Malmo, Sweden, June 2015.

 

1. “Glucose Oxidase Affects Laccase and Bilirubin Oxidase Direct Bioelectrocatalytic Cathodes”

Milton, R.D., Giroud, F., Thumser, A.E., Minteer, S.D. and Slade, R.C.T.

Presented at the 225th Electrochemical Society Meeting, Orlando, Florida, USA, 11th-15th May 2014.

 

 

Outreach activities

3. Seminar - “Flavin-based electron bifurcation in Methanococcus maripaludis”

Milton, R.D., Ruth, J.C., Gu, W., Deutzmann, J.S., Shima, S. and Spormann, A.M.

Advanced Seminar in Microbial Molecular Biology – Stanford University, March 2019.

 

2. Podcast contribution - “Chemistry in its element: Nitrogenase”.

Chemistry World, by the Royal of Society of Chemistry. Released on February 22nd 2019.

https://www.chemistryworld.com/podcasts/nitrogenase/3010143.article

 

1. Seminar - “Complex electron transfer mechanisms of metalloenzymes: Nitrogen fixation and electron bifurcation”

Milton, R.D.

Department of Chemistry, University of Cambridge, UK (August 7th, 2018).

**Invited seminar**

 

 

General contributions to science

1. Organizer and co-chair of the 2018 Gordon Research Seminar of Electrochemistry, January 2019 in California (USA). The PI organized a mentorship session within this postdoctoral and graduate student conference: “Finding a Scientific Career in Academia, Government Research and Industry”.