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Table of Contents
Labs
Safety rules
Things to check before you leave
Lipid handling
How to make aliquots
[[SOP aliquot]]
[[SOP aliquot for Photopolymerizable lipids]]
Vesicle fabrication by extrusion
[[SOP vesicle]]
Easy Calculation for mixed lipid vesicles preparation
[[Molar fraction calc]]
Supported lipid bilayer formation
Lipid nanotube formation
report_master_thesis_kristina_jajcevic.pdf
GUV electroformation
[[SOP for GUV Electroformation]]
In Vitro
Hela Cell Culture
[[Hela Cell Culture]]
MDCK Cell Culture
[[MDCK Cell Culture]]
Loading Cells with Caged Fluorescein
[[Loading HeLa Cells with Caged Fluorescein]]
Others
SOP for conductance experiment in free-standing lipid bilayers
conductance_experiment_protocol.pdf
SOP for chloriding silver wires
[[SOP for chloriding silver wires]]
Buffer making
[[HEPES buffer]]
Piranha solution
[[SOP Piranha]]
Check real purity for chemicals ordered via www.sigmaaldrich.com
Sigma
ITC for peptide-vesicle interactions
[[ITC Book Chapter]]
[[ITC experiment]]
[[ITC official guide]]
FRAP
[[SOP FRAP]]