highlight 10 2017

We have identified two contrast mechanisms for visualizing lateral pressure and ordering in phospholipid membranes using a mechanosensor probe:

1) The interface-specific Second Harmonic Generation (SHG) technique reveals that the interfacial spectra of the probe are shifted to lower energy only in ordered monolayers (figure A).

2) A second mechanism uses polarization-resolved SHG measurements, which give access to the average orientation of the mechanosensor (figure B). Different monolayers at different surface pressures can be differentiated according to the average orientation of the probe.

These two SHG-based approaches can be used in the future for real-time imaging of biological samples, complementing fluorescence methods.

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Eric Vauthey

Physical Chemistry Department - Sciences II - University of Geneva
30, Quai Ernest Ansermet - CH-1211 Geneva 4 (Switzerland)


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