highlight 12 2021

Oxazole yellow (YO) dyes are well-known fluorescent DNA probes. They are essentially non-emissive in solution but become strongly fluorescent upon binding to DNA. To better understand the early excited-state dynamics of YO dyes, we are applying broadband fluorescence up-conversion spectroscopy with sub-100 fs resolution. The figures show time-resolved fluorescence spectra recorded with a YO dye in aqueous solution with and without DNA and in ethanol. In the presence of DNA, the decay is slow and little spectral dynamics is observed, in agreement with the inhibition of non-radiative decay via large-amplitude motion. Without DNA, the fluorescence lifetime is smaller than 5 ps and significant spectral dynamics is visible. Its origin is presently under study.

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Eric Vauthey

Physical Chemistry Department - Sciences II - University of Geneva
30, Quai Ernest Ansermet - CH-1211 Geneva 4 (Switzerland)

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