IMAGE OF THE MONTH
Yamini Ravichandran, a postdoctoral fellow in the laboratory of Aurélien Roux (Biochemistry) created this video of a two headed morphogenetic defective Hydra expressing GFP-LifeAct, showing the apical surface and the supracellular actin cables running along the animal.
This volumetric rendering was done at the Photonic Bioimaging Center, using Imaris software on 3D data acquired on our SP8Dive Multiphoton Confocal Microscope.
It is dedicated to providing state-of-the-art equipment and technology for light microscopy. Specialists offer advice and guidance for each step of your imaging project starting from experimental approach to data analysis.
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- Advice for experimental approach and design for cell imaging
- Guidance for sample preparation (immunostaining or in situ hybridization)
- Supervision and guidance for the use of the confocal and widefield fluorescence microscopes
- Training : New users are trained on the instruments until they become independent
- Data analysis and quantification using free (ImageJ, FIJI) and dedicated
commercial software (Imaris, Huygens)
The center is equipped with bench space that is available to users of our service.
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- Design and optimization of protocols for complex experiments and applications (especially living-cell conditions, FRET, FRAP, superresolution)
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