BioCode: RNA to proteins (R2P) Core Facility
BioCode: RNA to proteins (R2P) Core Facility analyzes gene expression at the level of translation.
We provide analysis of translatome by polysome fractionation and Ribo-Seq (differential translation, translation efficiency, RNA translatability, ribosome pausing, etc).
We also perform in vitro translation in cell-free protein system and analysis of protein solubility.
We are working with different organisms and tissue cultures: bacteria, pathogens, yeast, mammalian cells and tissues...
We provide consulting, assistance and technical advice to help researcher in experiments design.
Our Facility is dedicated to local academic users (University of Geneva) but is also open for external academic researchers and for industry.
Equipment
- Density Gradient Fractionation System (BioComp)
- Density Gradient Fractionation System (Isco)
- Gradient Master
- Cryogenic cell/tissue mill
- Luminometer
Services
- Ribosome isolation and fractionation
- Analysis of translatome by Ribo-Seq (Ribosome profiling)
- Cryogenic Disruption
- Analysis of protein solubility
- Isolation of soluble and aggregated proteins
- Density gradient centrifugation
- In vitro translation in cell-free protein systems (CFPS)
Address
Centre Médical Universitaire (CMU)
1 rue Michel-Servet
1211 Genève 4