RE.A.D.S Unit
The READS unit (REaders, Assay Development & Screening unit) of the Faculty of Medicine provides access to different types of readers and material of screening.
It provides support for assay development in all types of formats: from single-point 96 and/or 384 well plates as well as data analysis.
Screening projects are welcome and a library of approximately 15,000 chemical compounds is available for high throughput screening (HTS). All types of nonradioactive assays are feasible at the READS unit: single point or kinetic measurements for absorbance, fluorescence and luminescence, such as ELISA, Ca2+, ROS, luciferase activity, oxygen consumption among others.
The platform is accessible to researchers of the University of Geneva and external users (academia and start-ups).
Equipment
Multi-mode microplate readers
- Fluostar Optima (BMG)
- Paradigm Spectramax (Molecular devices) (2X)
- Hidex Sense (Labgene)
Fluorescent/luminescent plate imager: FDSS µCell (Hamamatsu Photonics)
Infrared imaging system: Odyssey (LI-COR)
Real-time cell metabolic analysis: Seahorse XF96 Analyser
Immunospot analyser: Elispot / Fluorospot (Mabtech)
Cell imaging multi-mode reader: Cytation 5 (Biotek)
Automated Live Cell Analysis: ICelligence (RTCA S16) (OLS)
Liquid handlers
- Zephyr (Perkin Elmer)
- Viaflow (Integra)
- Assist+ (Integra)
Automated workstation: Fluent780 (Tecan)
Services
- Availability of advanced readers for single-point and kinetic measurements.
- Access to a chemical library of approximately 15,000 compounds for HTS.
- Support for multiple assay formats (e.g., 96- or 384-well plates).
- Expertise in designing and running assays for different molecular and cellular readouts, such as calcium signalling, reactive oxygen species, luciferase activity, and oxygen consumption.
