Facilities

SDSL EPR techniques routinely used in the lab for protein science:

1. CW EPR at physiological temperature to obtain dynamics of the spin-labeled side chain
2. ODNP at physiological temperature to measure directly water accessibility towards a particular spin-labeled site, therefore topology of the protein with respect to the membrane-water interface
3. Dipolar spectroscopy, in particular DEER to extract interspin distances between spin-labeled sites, therefore intra- and inter-protein interactions. Orthogonal labels allow multiple interactions to be probed at once.