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Publication 121  

  1. Nucleic Acid Templated Chemical Reaction in a Live Vertebrate
    L. Holtzer, I. Oleinich, M. Anzola, E. Lindberg, K. K. Sadhu, M. Gonzalez-Gaitan, N. Winssinger
    ACS Cent. Sci. 2016, 2, 394-400

Nucleic acid templated reactions are enabled by the hybridization of probe-reagent conjugates resulting in high effective reagent concentration and fast chemical transformation. We have developed a reaction that harnesses cellular microRNA (miRNA) to yield the cleavage of a linker releasing fluorogenic rhodamine in a live vertebrate. The reaction is based on the catalytic photoreduction of an azide by a ruthenium complex. We showed that this system reports specific expression of miRNA in living tissues of a vertebrate.

DOI: 10.1021/acscentsci.6b00054 

open archive unige:84737