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Publication 125  

  1. Allosterically Regulated Phosphatase Activity from Peptide-PNA Conjugates Folded Through Hybridization
    T. Machida, S. Dutt, N. Winssinger
    Angew. Chem. Int. Ed. 2016, 55, 8595-8598

The importance of spatial organization in short peptide catalysts is well recognized. We synthesized and screened a library of peptides flanked by peptide nucleic acids (PNAs) such that the peptide would be constrained in a hairpin loop upon hybridization. A screen for phosphatase activity led to the discovery of a catalyst with >25-fold rate acceleration over the linear peptide. We demonstrated that the hybridization-enforced folding of the peptide is necessary for activity, and designed a catalyst that is allosterically controlled using a complementary PNA sequence.

DOI: 10.1002/anie.201602751 

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