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Publication 136  

  1. Accelerating Turnover Frequency in Nucleic Acid Templated Reactions
    D. Chang, K. T. Kim, E. Lindberg, N. Winssinger
    Bioconjugate Chem. 2018, 29, 158-163

Nucleic acid templated reactions have attracted attention as an important technology to sense oligonucleotides and to translate nucleic acid-based instructions into diverse outputs. Great progresses have been made in accelerating the reaction in order to improve signal amplification, reaching rates where substrate turn-over rather than chemical reaction is rate limiting. Herein we explore the utility of architectures inspired by three-way junction that yield a cleavage of a strand thus accelerating substrate turn-over. We demonstrate that such design can overcome product inhibition in templated reactions and operate close to the rate of hybridization.

DOI: 10.1021/acs.bioconjchem.7b00663 

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