149. Luciferase-Induced Photouncaging: Bioluminolysis
     D. Chang, E. Lindberg, S. Feng, S. Angerani, H. Riezman, N. Winssinger
     Angew. Chem. Int. Ed. 2019, 58, 16033-16037

Bioluminescence resonance energy transfer (BRET) has been widely used for studying dynamic processes in biological systems such as protein–protein interactions and other signaling events. Aside from acting as a reporter, BRET can also turn on functions in living systems. Herein, we report the application of BRET to performing a biorthogonal reaction in living cells; namely, releasing functional molecules through energy transfer to a coumarin molecule, a process termed bioluminolysis. An efficient BRET from Nanoluc-Halotag chimera protein (H-Luc) to a coumarin substrate yields the excited state of coumarin, which in turn triggers hydrolysis to uncage a target molecule. Compared to the conventional methods, this novel uncaging system requires no external light source and shows fast kinetics (t_1/2

DOI : 10.1002/anie.201907734 

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