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Publication 60  

  1. Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes
    Z. Pianowski, K. Gorska, L. Oswald, C. A. Merten, N. Winssinger
    J. Am. Chem. Soc. 2009, 131, 6492-6497

Nucleic acid-templated reactions leading to a fluorescent product represent an attractive strategy for the detection and imaging of cellular nucleic acids. Herein we report the use of a Staudinger reaction to promote the reduction of profluorescent azidorhodamine. The use of two cell-permeable GPNA probes, one labeled with the profluorescent azidorhodamine and the other with trialkylphosphine, enabled the detection of the mRNA encoding O-6-methylguanine-DNA methyltransferase in intact cells.

DOI: 10.1021/ja809656k 

open archive unige:24717