Selection of publications

Nef google scholar


Ademi H, Djari C, Mayère C, Neirijnck Y, Sararols P, Rands CM, Stévant I, Conne B, Nef S.
Deciphering the origins and fates of steroidogenic lineages in the mouse testis.
Cell Rep. 2022 Jun 14;39(11):110935. doi: 10.1016/j.celrep.2022.110935.

Mayère C, Regard V, Perea-Gomez A, Bunce C, Neirijnck Y, Djari C, Bellido-Carreras N, Sararols P, Reeves R, Greenaway S, Simon M, Siggers P, Condrea D, Kühne F, Gantar I, Tang F, Stévant I, Batti L, Ghyselinck NB, Wilhelm D, Greenfield A, Capel B, Chaboissier MC, Nef S.
Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad.
Sci Adv. 2022 May 27;8(21):eabm0972. doi: 10.1126/sciadv.abm0972. Pubmed

Rahban R, Rehfeld A, Schiffer C, Brenker C, Egeberg Palme DL, Wang T, Lorenz J, Almstrup K, Skakkebaek NE, Strünker T, Nef S.
The antidepressant Sertraline inhibits CatSper Ca2+ channels in human sperm
Hum Reprod. 2021 Sep 18;36(10):2638-2648. doi: 10.1093/humrep/deab190Pubmed

Sararols P, Stévant I, Neirijnck Y, Rebourcet D, Darbey A, Curley MK, Kühne F, Dermitzakis E, Smith LB, Nef S.
Specific transcriptomic signatures and dual regulation of steroidogenesis between fetal and adult mouse Leydig cells.
Front Cell Dev Biol. 2021 Jun 28;9:695546. doi: 10.3389/fcell.2021.695546. eCollection 2021Pubmed

Mayère C, Neirijnck Y, Sararols P, Rands CM, Stévant I, Kühne F, Chassot AA, Chaboissier MC, Dermitzakis ET, Nef S.
Single- cell transcriptomics reveal temporal dynamics of critical regulators of germ cell fate during mouse sex determination
FASEB J. 2021 Apr;35(4):e21452. doi: 10.1096/fj.202002420RPubmed

Istvan M, Rahban R, Dananche B, Senn A, Stettler E, Multigner L, Nef S, Garlantézec R. 
Maternal occupational exposure to endocrine-disrupting chemicals during pregnancy and semen parameters in adulthood: results of a nation wide cross-sectional study among Swiss conscripts
Hum Reprod. 2021 Jun 18;36(7):1948-1958. doi: 10.1093/humrep/deab034Pubmed

Rahban R, Nef S. 
Regional difference in semen quality of young men: a review on the implication of environmental and lifestyle factors during fetal life and adulthood
Basic Clin Androl. 2020 Oct 15;30:16. doi: 10.1186/s12610-020-00114-4Pubmed

Rahban R, Nef S. 
CatSper: The complex main gate of calcium entry in mammalian spermatozoa.
Mol Cell Endocrinol. 2020 Dec 1;518:110951. doi: 10.1016/j.mce.2020.110951. Pubmed

Neirijnck Y, Papaioannou MD, Nef S 
The Insulin/IGF System in Mammalian Sexual Development and Reproduction.
Int J Mol Sci. 2019 Sep 9;20(18):4440. doi: 10.3390/ijms20184440. Pubmed

Rahban R, Priskorn L, Senn A, Stettler E, Galli F, Vargas J, Van den Bergh M, Fusconi A, Garlantezec R, Jensen TK, Multigner L, Skakkebaek NE, Germond M, Jørgensen N, Nef S 
Semen quality of young men in Switzerland: a nationwide cross-sectional population-based study.
Andrology. 2019 May 21. doi: 10.1111/andr.12645. Pubmed

Stevant I, Kuhne F, Greenfield A, Chaboissier M-C, Dermitzakis ET Nef S
Dissecting cell lineage specification and sex fate determination in gonadal somatic cells using single-cell transcriptomics. 
Cell Reports, 2019, March 19;26(12):3272-3283.e3. Pubmed

Stévant I & Nef S.

Genetic control of gonadal sex determination and development.
Trends in Genetics. Review.2019, March 19. pii: S0168-9525(19)30033-2. Pubmed

Nef S, Stévant I & Greenfield A.
Characterizing the bipotential mammalian gonad.
Current Topics in Developmental Biology. Review.2019;134:167-194. Pubmed

Neirijnck Y, Kühne F, Mayère C, Pavlova E, Sararols P, Foti M, Atanassova N, Nef S
Tumor suppressor PTEN regulates negatively Sertoli cell proliferation, testis size, and sperm production in vivo.
Endocrinology. 2019 Feb 1;160(2):387-398. Pubmed

Stévant I, Papaioannou MD, Nef S.

A brief history of sex determination.
Mol Cell Endocrinol. 2018 Apr 7. Review. Pubmed

Stévant I and Nef S..
Single cell transcriptome sequencing: a new approach for the study of mammalian sex determination. 
Mol Cell Endocrinol,  2018 Jan 26. Review. Pubmed

Stévant I, Neirjinck Y, Borel C, Escoffier J, Smith LB, Antonarakis SE, Dermitzakis ET, Nef  S
Deciphering cell lineage specification during male sex determination with single-cell RNA sequencing. 
Cell Reports 2018 Feb 6. Pubmed

Neirjinck Y, Calvel P, Kilcoyne K , Kühne F, Stévant I, Griffeth RJ, Pitetti JL, Andric S, Pralong F, Smith LB & Nef S. 
Insulin and IGF1 receptors are essential for the development and steroidogenic function of adult Leydig cells. 
FASEB J, 2018 Jan 24 . Pubmed

Stévant I and Nef S. 
Single cell transcriptome sequencing: a new approach for the study of mammalian sex determination.
Mol Cell Endocrinol, 2018 Jan 26. Pubmed

Zimmermann C, Stévant I, Borel C, Conne B, Pitetti JL, Calvel P, Kaessmann H, Jégou B, Chalmel F, Nef S
Research Resource: The dynamic transcriptional profile of Sertoli cells during the progression of spermatogenesis.
Molecular Endocrinology, 2015 Feb 24. Pubmed

Céline Zimmermann, Yannick Romero, Maria Warnefors, Adem Bilican, Christelle Borel, Lee B. Smith, Noora Kotaja, Henrik Kaessmann, Serge Nef
Germ Cell-Specific Targeting of DICER or DGCR8 Reveals a Novel Role for Endo-siRNAs in the Progression of Mammalian Spermatogenesis and Male Fertility.
PLoS One, 2014 Sep 22. Pubmed

Patrick Callier, Pierre Calvel, Armine Matevossian, Periklis Makrythanasis, Pascal Bernard, Hiroshi Kurosaka, Anne Vannier, Christel Thauvin-Robinet, Christelle Borel, Séverine Mazaud-Guittot, Antoine Rolland, Christèle Desdoits-Lethimonier, Michel Guipponi, Céline Zimmermann, Isabelle Stévant, Françoise Kuhne, Béatrice Conne, Federico Santoni, Sandy Lambert, Frederic Huet, Francine Mugneret, Jadwiga Jaruzelska, Laurence Faivre, Dagmar Wilhelm, Bernard Jégou, Paul A. Trainor, Marilyn D. Resh, Stylianos E. Antonarakis, Serge Nef
Loss of Function Mutation in the Palmitoyl-Transferase HHAT Leads to Syndromic 46,XY Disorder of Sex Development by Impeding Hedgehog Protein Palmitoylation and Signaling.
PLoS Genetics, 2014 May 1st. Pubmed

Magali Soumillon M., Necsulea A., Weier, M., Brawand D., Zhang X., Gu H., Barthès P., Kokkinaki M., Nef S., Gnirke A., Dym M., de Massy B., Mikkelsen TS., Kaessmann H.
Cellular source and mechanisms of high transcriptome complexity in the mammalian testis.
Cell Reports, 2013 Jun 19. Pubmed

Pitetti JL, Calvel P, Zimmermann C, Conne B, Papaioannou MD, Aubry F, Cederroth C, Urner F, Fumel B, Crausaz M, Docquier M, Herrera PL, Pralong F, Germond M, Guillou F, Jégou B, Nef S.
An essential role for insulin and IGF1 receptors in regulating Sertoli cells proliferation, testis size and FSH action in mice.
Molecular Endocrinology. 2013 May;27(5):814-27  Pubmed

Pitetti JL, Calvel P, Romero Y, Conne B, Truong V, Papaioannou MD, Schaad O, Docquier M, Herrera PL,Wilhelm D and Nef S.
Insulin and IGF1 Receptors Are Essential for XX and XY Gonadal Differentiation and Adrenal Development in Mice.
PLoS Genetics, 2013 Jan;9(1):e1003160. Pubmed

Romero Y., Vuandaba M., Suarez P., Grey C., Calvel P., Conne B., Pearce D., de Massy B., Hummler E., Nef S.
The Glucocorticoid-Induced Leucine Zipper (GILZ) Is Essential for Spermatogonial Survival and Spermatogenesis.
Sex Dev. 2012 May, 6(4):169-77. Pubmed

Romero Y, Meikar O, Papaioannou MD, Conne B, Grey C, Weier M, Pralong F, de Mass B, Kaessmann H, Vassalli JD, Kotaja N, Nef S.
Dicer1 depletion in male germ cells leads to infertility due to cumulative meiotic and spermiogenic defects.
PLoS One, 2011;6(10):e25241. Pubmed

Papaioannou MD, Lagarrigue M, Vejnar CE, Rolland AD, Kühne F, Aubry F, Schaad O, Fort A, Descombes P, Neerman-Arbez M, Guillou F, Zdobnov EM, Pineau C and Nef S.
Loss of Dicer in Sertoli cells has a major impact on the testicular proteome of mice.
Molecular and Cellular Proteomics, 2011 Apr;10(4) . Pubmed

Grad I., Cederroth C.R., Walicki J., Grey C., Barluenga S., Winssinger N., De Massy B., Nef S., and Didier Picard D.
The molecular chaperone Hsp90alpha is required for meiotic progression of spermatocytes beyond pachytene in the mouse.
PLoS One, 2010 Dec 31;5(12):e15770 . Pubmed

Cederroth C.R., Zimmermann C., Beny J.-L., Schaad O., Combepine C., Descombes P., Doerge D.R., Pralong F.P., Vassalli J.-D., Nef S.
Potential detrimental effects of a phytoestrogen-rich diet on male fertility in mice.
Mol Cell Endocrinol, 2010 Jun 10;321(2):152-60. Pubmed

Swetloff A., Conne B., Huarte J., Pitetti J.-L., Nef S., Vassalli J.-D.
DCP1-bodies in mouse oocytes.
Mol Cel Biol, 2009 Dec;20(23):4951-61. Pubmed

Pitetti J.-L.,Torre D., Conne B., Papaioannou M.D., Cederroth C.R., Xuan S., Kahn R., Parada L.F., Vassalli J.-D., Efstratiadis A. and Nef S.
IR and IGF1R are not required for oocyte growth, differentiation and maturation in mice.
Sex Dev, 2009;3(5):264-72. Pubmed

Papaioannou MD, Pitetti JL, Ro S, Park C, Aubry F, Schaad O, Vejnar CE, Descombes P, Zdobnov EM, McManus MT, Guillou F, Harfe BD, Yan W, Jégou B, Nef S.
Sertoli cell Dicer is essential for spermatogenesis in mice.
Developmental Biology, 2009 Feb 1;326(1):250-9. Pubmed

Cederroth C.R., Schaad O., Descombes P., Changeux P., Vassalli, J.-D., Nef S.
ERα is a major contributor of estrogen-mediated testis dysgenesis and cryptorchidism.
Endocrinology. 2007 Nov;148(11):p.5507-5519 . Pubmed

Nef S, Schaad O, Stallings NR, Cederroth CR, Pitetti JL, Schaer G, Malki S, Dubois-Dauphin M, Boizet-Bonhoure B, Descombes P, Parker KL, Vassalli JD.
Gene expression during sex determination reveals a robust female genetic program at the onset of ovarian development.
Developmental Biology, 2005 Oct., 287: 361-377 .Pubmed

Nef S, Verma-Kurvari S, Merenmies J, Vassalli JD, Efstratiadis A, Accili D, and Parada LF.
Testis determination requires insulin receptor family function in mice.
Nature, 2003, November 20;426 (6964):291-5. Pubmed

Nef S, Shipman T, Parada LF.
Molecular basis for estrogen-induced cryptorchidism.
Developmental Biology, vol. 224 (August 2000), p.354-361 . Pubmed

Nef S. and Parada LF.
Cryptorchidism in Insl3 mutant mice.
Nature Genetics, vol 22, p.295-299, July 1999. Pubmed