Electron microscopy (EM) provides unprecendeted access to study and analyze biological samples at the ultrastructural level, with resolution in nanometers scale.
EM is notably suitable for:
- visualizing normal and abnormal cellular and tissue morphology
- analyzing the intracellular localization of proteins, lipids and other macromolecules
- reconstructing the 3D ultrastructure of cells and tissues
The Electron Microscopy Facility (Pôle Facultaire de Microscopie Ultrastructurale (PFMU)) at the Medical Faculty of the University of Geneva:
- is an open door multi-user facility performing most aspects of biological research that require the use of scanning and/or transmission electron microscopy (SEM/TEM).
- anyone in the University research community who needs to use the PFMU facilities is welcome to have an access to facility infrastructure
- PFMU is also open to users from other universities and from industry.
- PFMU spacializes for cellular electron microscopy (TEM and SEM) using conventional chemical fixation for:
- ultramicrotomy and ultra-thin sectioning for TEM analysis
- cryo-ultramicrotomy and immuno-gold labelling
- volume imaging using focused ion beam and SEM imaging (FIBSEM) for 3D reconstruction of the cells or tissues
- SEM imaging
- negative staining
To cover part of the costs and to be able to maintain the equipment, fees to use the equipment and fees for specimen preparation are charged to the users.
A prospective user should contact a member of the PFMU staff (see contacts) and determine if the instruments in the facility are suitable for the proposed research.