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Electron microscopy (EM) provides unprecendeted access to study and analyze biological samples at the ultrastructural level, with resolution in nanometers scale.

EM is notably suitable for:

  • visualizing normal and abnormal cellular and tissue morphology
  • analyzing the intracellular localization of proteins, lipids and other macromolecules
  • reconstructing the 3D ultrastructure of cells and tissues

 

The Electron Microscopy Facility (Pôle Facultaire de Microscopie Ultrastructurale (PFMU)) at the Medical Faculty of the University of Geneva:

  • is an open door multi-user facility performing most aspects of biological research that require the use of scanning and/or transmission electron microscopy (SEM/TEM).
  • anyone in the University research community who needs to use the PFMU facilities is welcome to have an access to facility infrastructure
  • PFMU is also open to users from other universities and from industry.
  • PFMU specializes for cellular electron microscopy (TEM and SEM) using conventional chemical fixation for:
    • ultramicrotomy and ultra-thin sectioning for TEM analysis
    • cryo-ultramicrotomy and immuno-gold labelling
    • volume imaging using focused ion beam and SEM imaging (FIBSEM) for 3D reconstruction of the cells or tissues
    • SEM imaging
    • negative staining
    • ...

 

To cover part of the costs and to be able to maintain the equipment, fees to use the equipment and fees for specimen preparation are charged to the users.

 

Initial contact

A prospective user should contact a member of the PFMU staff (see contacts) and determine if the instruments in the facility are suitable for the proposed research.