Bohumil Maco, Ph.D.

Scientific supervisor (70%)

+41 22 379 53 05

Since 10/2022 Scientific Supervisor of the Electron Microscopy Facility (PFMU) at the Medical Faculty, University of Geneva, Geneva, Switzerland
10/2016 – 09/2022 Senior Scientist in the lab of Prof. Dominique Soldati-Favre at the Department of Microbiology and Molecular Medicine, Medical Faculty, University of Geneva, Geneva, Switzerland
07/2015 – 09/2016 Post-doctoral scientist in the laboratory of Prof. Anders Meibom at EPFL, Lausanne, Switzerland
05/2014 – 06/2015 Post-doctoral scientist in the laboratory of Prof. Hilal Lashuel at the Brain Mind Institute, EPFL, Lausanne, Switzerland
02/2011 – 04/2014 Post-doctoral scientist in the laboratory of Prof. Graham Knott at the BioEM facility, EPFL, Lausanne, Switzerland
03/2010 – 12/2010 Post-doctoral scientist in the laboratory of Prof. Leann Tilley at the School of Molecular Sciences, La Trobe University, Melbourne, Australia
08/2006 – 09/2009 Post-doctoral scientist in the laboratory of Prof. Bostjan Kobe at the School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia
02/2001 – 08/2006 Post-doctoral scientist in the laboratory of Prof. Ueli Aebi at the Maurice E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Switzerland
09/1996 – 11/2000

Ph.D. in Biology and Animal Physiology, in the Laboratory of Electron Microscopy at the Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava, Slovak Republic

09/1991 – 06/1996 M.Sc. in Biology and Cytology at the Comenius University, Faculty of Natural Science, Bratislava, Slovak Republic
  • Image Processing and Analysis module MBM17: Life Sciences PhD School Geneva (June 2021-23)

Application of image processing and analysis in electron microscopy focussed on analysis of the 3D volume datasets acquired by serial sections TEM and FIBSEM (image alignment, segmentation, generation of the 3D model, and visualization and renderring)

  • BioImaging module MBM11 (Imaging Techniques in Life and Biomedical Sciences): Life Sciences PhD School Geneva (June 2022-23)

General principles of electron microscopy techniques for TEM, SEM, FIBSEM and for correlative light and electron microscopy and afternoon the practical demonstration of same sample preparations and examination with TEM microscope



  1. Pietsch E., Ramaprasad A., Bielfeld S., Wohlfarter Y., Maco B., Niedermüller K., Wilcke L., Kloehn J., Keller M.A., Soldati-Favre D., Blackman M.J., Gilberger T.-W., Burda P.-C. (2023): A patatin-like phospholipase is important for mitochondrial function in malaria parasites. mBio: e0171823                  Online ahead of print.

  2. Spataro S.*, Maco B.*, Escrig S., Jensen L., Polerecky L., Knott G., Meibom A., Schneider B.L. (2023): Stable isotope labeling and ultra-high-resolution NanoSIMS imaging reveal alpha-synuclein-induced changes in neuronal metabolism in vivo. Acta Neuropathol. Commun. 11(1): 157                     * Contributed equally

  3. Köhler T., Luscher A., Falconnet L., Resch G., McBride R., Mai Q.A., Simonin J.L., Chanson M., Maco B., Galiotto R., Riat A., Civic N., Docquier M., McCallin S., Chan B., van Delden C. (2023) Personalized aerosolised bacteriophage treatment of a chronic lung infection due to multidrug-resistant Pseudomonas aeruginosa. Nat. Commun. 14(1): 3629

  4. Dubois D.J., Chehade S., Marq J.B., Venugopal K., Maco B., Tell I Puig A., Soldati-Favre D., Marion S. (2023) Toxoplasma gondii HOOK-FTS-HIP complex is critical for secretory organelle discharge during motility, invasion, and egress. mBio. 14(3): e0045823

  5. Kuehnel R.M., Ganga E., Balestra A.C., Suarez C., Wyss M., Klages N., Brusini L., Maco B., Brancucci N., Voss T.S., Soldati-Favre D., Brochet M. (2023) A Plasmodium membrane receptor platform integrates cues for egress and invasion in blood forms and activation of transmission stages. Sci. Adv. 9(24): eadf2161


2016 2022:

  1. Dogga S.K., Lunghi M., Maco B., Li J., Marq J.B., Chicherova N., Kockmann T., Bumann D., Hehl A.B., Soldati-Favre D., Hammoudi P.M. (2022) Importance of aspartyl protease 5 in the intracellular niche during acute and chronic infection of Toxoplasma gondii. Mol. Microbiol. 118(6): 601-622

  2. Dos Santos Pacheco N., Brusini L., Haase R., Tosetti N., Maco B., Brochet M., Vadas O., Soldati-Favre D. (2022) Conoid extrusion regulates glideosome assembly to control motility and invasion in Apicomplexa. Nat. Microbiol. 7(11): 1777-1790

  3. Nyonda M.A., Kloehn J., Sosnowski P., Krishnan A., Lentini G., Maco B., Marq J.B., Hannich J.T., Hopfgarter G., Soldati-Favre D. (2022) Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii. Cell Rep. 40(7): 111224

  4. Dos Santos Pacheco N., Tosetti N., Krishnan A., Haase R., Maco B., Suarez C., Ren B., Soldati-Favre D. (2021) Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complex. mBio. 12(5): e0205721

  5. Lentini G., Ben Chaabene R., Vadas O., Ramakrishnan C., Mukherjee B., Mehta V., Lunghi M., Grossmann J., Maco B., Visentin R., Hehl A.B., Korkhov V.M., Soldati-Favre D. (2021) Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion. Nat. Commun. 12(1): 3788

  6. Bertiaux E., Balestra A.C., Bournonville L., Louvel V., Maco B., Soldati-Favre D., Brochet M., Guichard P., Hamel V. (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biol. 19(3): e3001020

  7. Bisio H., Ben Chaabene R.B., Sabitzki R., Maco B., Marq J.B., Gilberger T.W., Spielmann T., Soldati-Favre D. (2020) The ZIP code of vesicle trafficking in Apicomplexa: SEC1/Munc18 and SNARE proteins. mBio. 11(5): e02092-20

  8. Tosetti N., Dos Santos Pacheco N., Bertiaux E., Maco B., Bournonville L., Hamel V., Guichard P., Soldati-Favre D. (2020) Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii. eLife 9: e56635

  9. Lentini G., Dubois D.J., Maco B., Soldati-Favre D., Frénal K. (2019) The role of Centrin 2 and Dynein Light Chain 8a in apical secretory organelles discharge of Toxoplasma gondii. Traffic 20(8): 583-600

  10. Fang H., Gomes A.R., Klagen N., Pino P., Maco B., Walker E.M., Zenonos Z.A., Angrisano F., Baum J., Doerig C., Baker D.A., Bilker O., Brochet M. (2018) Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites. Nat. Commun. 9(1): 4248

  11. Cali C., Wawrzyniak M., Becker C., Maco B., Cantoni M., Jorstad A., Nigro B., Grillo F., De Paola V., Fua P., Knott G.W. (2018) The effects of aging on neuropil structure in mouse somatosensory cortex – A 3D electron microscopy analysis of layer 1. PLoS One 13(7): e0198131

  12. Hammoudi P.M., Maco B., Dogga S.K., Frénal K., Soldati-Favre D. (2018) Toxoplasma gondii TFP1 is an essential transporter family protein critical for microneme maturation and exocytosis. Mol. Microbiol. 109(2): 225-244

  13. Pino P., Caldelari R., Mukherjee B., Vahokoski J., Klages N., Maco B., Collins C.R., Blackman M.J., Kursula I., Heussler V., Brochet M., Soldati-Favre D. (2017) A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress. Science 358(6362): 522-528

  14. Frénal K., Jacot D., Hammoudi P.M., Graindorge A., Maco B., Soldati-Favre D. (2017) Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii. Nat. Commun. 8: 15710


1997 2016:

  1. Fares M.B., Maco B., Oueslati A., Rockenstein E., Ninkina N., Buchman V.L., Masliah E., Lashuel H.A. (2016) Induction of de novo α-synuclein fibrillization in a neuronal model for Parkinson’s disease. Proc. Natl. Acad. Sci. U. S. A. 113(7): E912-E921

  2. Benmessaoud, I.R., Mahul-Mellier A.L., Horváth E., Maco B., Spina M.,Lashuel H.A., Forró L. (2016) Health hazard of methylammonium lead iodide based perovskites: cytotoxicity studies. Toxicol. Res. 5(2): 407-419

  3. Mahul-Mellier A.L., Vercruysse F., Maco B., Ait-Bouziad N., De Roo M., Muller D., Lashuel H.A. (2015) Fibril growth and seeding capacity play key roles in α-synuclein-mediated apoptotic cell death. Cell Death Differ. 22(12): 2107-2122

  4. Maco B., Holtmaat A., Jorstad A., Fua P., Knott G.W. (2014) Correlative in vivo 2-photon imaging and focused ion beam scanning electron microscopy: 3D analysis of neuronal ultrastructure. Methods Cell Biol. 124: 339-361 in: Correlative Light and Electron Microscopy II (T. Müller-Reichert and P. Verkade eds.) Elsevier Inc.

  5. Maco B., Cantoni M., Holtmaat A., Kreshuk A., Hamprecht F.A., Knott G.W. (2014) Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites. Nat. Protoc. 9(6): 1354-1366

  6. Cane M., Maco B., Knott G., Holtmaat A. (2014) The relationship between PSD-95 clustering and spine stability in vivo. J. Neurosci. 34(6): 2075-2086

  7. Canty A.J., Huang L., Jackson J.S., Little G.E., Knott G., Maco B., De Paola V. (2013) In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits. Nat Commun. 4: 2038

  8. Allegra Mascaro A.L., Cesare P., Sacconi L., Grasselli G., Mandolesi G., Maco B., Knott G.W., Huang L., De Paola V., Strata P., Pavone F.S. (2013) In vivo single branch axotomy induces GAP-43-dependent sprouting and synaptic remodeling in cerebellar cortex. Proc. Natl. Acad. Sci. U. S. A. 110(26): 10824-10829

  9. Grillo F.W., Song S., Teles-Grilo Ruivo L.M., Huang L., Gao G., Knott G.W., Maco B., Ferretti V., Thompson D., Little G.E., De Paola V. (2013) Increased axonal bouton dynamics in the aging mouse cortex. Proc. Natl. Acad. Sci. U. S. A. 110(16): E1514-E1523

  10. Maco B., Holtmaat A., Cantoni M., Kreshuk A., Straehle C.N., Hamprecht F.A., Knott G. (2013) Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons. PLoS One 8(2): e57405

  11. Mostany, R., Anstey J.E., Crump K.L., Maco B., Knott G., Portera-Cailliau C. (2013) Altered synaptic dynamics during normal brain aging. J. Neurosci. 33(9): 4094-4104

  12. Maiorca M., Hanssen E., Kazmierczak E., Maco B., Kudryashev M., Hall R., Quiney H., Tilley L. (2012) Improving the quality of electron tomography image volumes using pre-reconstruction filtering. J. Struct. Biol. 180(1): 132-142

  13. Maco B., Ross, I.L., Landsberg M.J., Mouradov, D., Saunders, N.F.W., Hankamer, B., Kobe, B. (2011) Proteomics and electron microscopy survey of large assemblies in macrophage cytoplasm. Mol. Cell. Proteomics,  10(6): 10.1074/mcp.M111.008763, 1-9

  14. Yeoman J.A., Hanssen E., Maier A.G., Klonis N., Maco B., Baum J., Turnbull L., Whitchurch C.B., Dixon M.W., Tilley L. (2011) Tracking glideosome-associated protein 50 reveals the development and organization of the inner membrane complex of Plasmodium falciparum. Eukaryot. Cell 10(4): 556-564

  15. Frenkiel-Krispin D., Maco B., Aebi U., Medalia O. (2010) Structural analysis of a metazoan nuclear pore complex reveals a fused concentric ring architecture. J. Mol Biol. 395(3): 578-586

  16. Kobe B., Guncar G., Buchholz R., Huber T., Maco B. (2009) The many faces of platelet glycoprotein Ibα - thrombin interaction.  Curr. Prot. Pept. Sci. 10(6): 551-558

  17. Kobe B., Guncar G., Buchholz R., Huber T., Maco B., Cowieson N., Martin J.L., Marfori M., Forwood J.K. (2008). Crystallography and protein-protein interactions: biological interfaces and crystal contacts. Biochem. Soc. Trans. 36(6): 1438-1441

  18. Schwarz-Herion K., Maco B., Sauder U., Fahrenkrog B. (2007) Domain topology of the p62 complex within the 3-D architecture of the nuclear pore complex. J. Mol. Biol. 370(4): 796-806

  19. Maco B., Fahrenkrog B., Huang N.P., Aebi U. (2006) Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy. Methods Mol. Biol. 322: 273-287 in: Xenopus Protocols: Cell Biology and Signal Transduction (X.J. Liu ed.) Humana Press Inc., Totowa, NJ

  20. Schäfer T., Maco B., Petfalski E., Tollervey D., Böttcher B., Aebi U., Hurt E. (2006) Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit. Nature  441(7093): 651-655

  21. Meyer D.M., Crottet P., Maco B., Degtyar E., Cassel D., Spiess M. (2005) Oligomerization and dissociation of AP-1 adaptors are regulated by cargo signals and by ArfGAP1-induced GTP hydrolysis. Mol. Biol. Cell 16(10): 4745-4754

  22. Marino M., Svergun D.I.,  Kreplak L., Konarev P.V., Maco B., Labeit D., Mayans O. (2005) Poly-Ig tandems from I-band titin share extended domain arrangements irrespective of the distinct features of their modular constituents. J. Muscle Res. Cell Motil.  26(6-8): 355-365

  23. Kim K.T., Choi H.H., Steinmetz M.O., Maco B., Kammerer R.A., Ahn S.Y., Kim H.Z., Lee G.M., Koh G.Y. (2005) Oligomerization and multimerization is critical for angiopoietin-1 to bind and phosphorylate Tie2. J. Biol. Chem. 280(20): 20126-20131

  24. Maco B.*, Köser J.*, Aebi U., Fahrenkrog B. (2005) The nuclear pore complex becomes alive: new insights into its dynamics and involvement in different cellular processes. Atlas Genet. Cytogenet. Oncol. Haematol. February 2005               *authors contributed equally

  25. Nissan T.A., Galani K., Maco B., Tollervey D., Aebi U., Hurt E. (2004) A pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol. Cell 15(2): 295-301

  26. Kiewitz R., Acklin C., Schäfer B.W., Maco B., Uhrík B., Wuytack F., Erne P., Heizmann C.W. (2003) Ca2+ -dependent interaction of S100A1 with the sarcoplasmic reticulum Ca2+ -ATPase2a and phospholamban in the human heart. Biochem. Biophys. Res. Commun. 306(2): 550-557

  27. Fahrenkrog B., Maco B., Fager A.M., Köser J., Sauder U., Ullman K.S., Aebi U. (2002) Domain-specific antibodies reveal multiple-site topology of Nup153 within the nuclear pore complex. J. Struct. Biol.  140(1-3): 254-267

  28. Maco B., Mandinova A., Dürrenberger M., Schäfer B.W., Uhrík B., Heizmann C.W. (2001) Ultrastructural distribution of the S100A1 Ca2+-binding protein in the human heart. Physiol. Res.  50(6): 567-574

  29. Maco B., Uhrík B., Heizmann C.W. (2000) distribution of the Ca2+-binding S100A1 protein at different sarcomere lengths of slow and fast rat skeletal muscles. Gen. Physiol. Biophys. 19(2): 237-244

  30. Maco B., Brezová A., Schäfer B.W., Uhrík B., Heizmann C.W. (1997) Localization of the Ca2+-binding S100A1 protein in slow and fast skeletal muscles of the rat. Gen. Physiol. Biophys. 16(4): 373-377