Ribosome isolation and fractionation

• Cell culturing and sample preparation for ribosome fractionation
• Optimization of conditions for optimal polysome preparation
• Ribosome fractionation in sucrose gradient
• RNA and protein isolation from sucrose gradients

RNA isolation for RNA-Seq

• Cell culturing
• RNA isolation for libraries with QC (Ribo-Seq input)

Analysis of translatome by Ribo-Seq (Ribosome profiling)

• Cell culturing and sample preparation for Ribo-Seq
• Optimization of nuclease digestion
• Ribo-Seq library preparation with rRNA depletion

Cryogenic Disruption

• Cryogenic disruption of different biological material in liquid nitrogen or dry ice
• Suitable for yeast, Gram+ bacteria, plants, hard tissues...

Analysis of protein solubility

• Cell culturing and sample preparation
• Isolation of aggregated proteins
• Analysis of the protein by SDS-PAGE/Western blot

Density gradient centrifugation

• Preparation and running of different gradients to separate cells, organells, proteins and protein complexes

In vitro translation in cell-free protein systems

• Cell culturing
• Preparation of CFPS from customer cells
• In vitro transcription of mRNA transcripts, capping, polyA tailing
• Analysis of the protein production by SDS-PAGE/Western blot/luminescence

Analysis, Support and Training

• Standart, Custom & Advanced Analysis
• Support and consulting of our users
• Bioinformatic training