Axiocam Histo (Tel. 95 583)
Capabilities
This microscope is dedicated for imaging non fluorescently-labelled samples such as:
- fixed cell culture
- fixed isolated cells
- thin tissue sections (inclusion)
Detailed specifications
Microscope
- Zeiss Axioskop 2 plus
- Halogen light source for widefield visualization
Objectives
- EC Plan-Neofluar 1x/0.025
- Plan-Neofluar 2.5x/0.075
- Plan-Neofluar 5x/0.15
- Plan-NeoFluar 10x/0.30 Ph1
- Plan-Neofluar 20x/0.50 Ph2
- Plan-Neofluar 40x/0.75 Ph2
| Objective | Micron/ pixel value |
| 1x | 6.390 |
| 2.5x | 2.577 |
| 5x | 1.347 |
| 10x | 0.673 |
| 20x | 0.329 |
| 40x | 0.166 |
| 63x | 0.106 |
| 100x | 0.067 |
| 10x en 2600x2060 | 0.337 |
| 10x en 3900x3090 | 0.224 |
Mounted filter cubes
- UV excitation DAPI, Hoechst 33258, ...
BP 365/12, FT 395, LP 397 - BLUE excitation FITC, GFP, YFP, CFP,...
BP 450-490, FT 510, BP 515-565 - GREEN excitation TRITC, Texas-Red, DsRed1,...
BP 546/12, FT 580, LP 590
Sources of Illumination
- Light illumination is through a 12V halogen lamp 100W
- Condenser 0.9 for small objectif 1x 2.5x
- Condenser 0.9s with H, Ph1, Ph2, Ph3 and D
- UV illumination is through a high pressure mercury lamp HBO 50W.
Detector
Axiocam Color HRc (Zeiss)
Characteristics:
Effective pixels : 1300x1030, 8 and 12 bit/pixel
Color obtained by color mask
Pixel size: 6.7 µm
Binning 2x,3x,4x,5x
Electronic shutter (1ms to 40 s)
Images are recorder on a PC computer (Windows XP)
Computer
- Windows XP
- AxioVision 4.8.1.0