Faculté de médecine Faculté de médecine

Widefield Microscopy (4 systems)

Video time-lapse + Temp Controller + CO2 (Tel. 94 778)

Capabilities

  • Fluorescence/ transmission widefield microscope : DAPI, CFP, YFP, GFP, mCherry, Cy5
  • DIC for objectives 40x and 63x
  • Temperature, CO2 control
  • Time-lapse video-microscopy (live cells).
  • Imaging fixed samples (culture cells)
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Detailed specifications

Microscope

  • Zeiss Axio Observer Z1 with Definite Focus 2

Objectives

  • Achroplan  4x / 0.10 WD 11.1 mm
  • EC Plan-Neofluar 10x / 0.30, Ph1  WD 5.2 mm  (Definite Focus 2 compatible)
  • EC Plan-Neofluar 20x / 0.50, Ph2  WD 2.0 mm  (Definite Focus 2 compatible)
  • EC Plan-Apochromat 20x/0.8 WD 0.55 mm (Definite Focus 2 compatible)
  • Plan Apochromat 40x / 1.4 Oil DIC III (UV) VIS-IR  (Definite Focus 2 compatible)
  • Plan Apochromat 63x / 1.4 Oil DIC III (Definite Focus 2 compatible)

 

Objective

Axiocam 506 mono

micron/pixel

Camera Spot

micron/pixel
4x 1.1 2.129
4x optovar 1.6 x 0.713 1.380
10x 0.44  0.852
10x optovar 1.6x 0.285  0.552
20x 0.220  0.428
20x optovar 1.6x 0.143  0.277
40x 0.110  0.213
40x optovar 1.6x 0.071  
63x 0.069  0.135
63x optovar 1.6x 0.045  

 

Excitation with Colibri 7 type R[G/Y]CBV-UV :

  1. 385 / 30 nm DAPI, Hoechst 33342, Hoechst 33258, Alexa Fluor 350, Alexa Fluor 405, Indo-1, eBFP / BFP, eGFP (wt), True Blue
  2. 423 / 44 nm Pacific Blue, Lucifer Yellow, Alexa Fluor 433, eCFP, Cerulean
  3. 469 / 38 nm FM1-43, Cy2, eGFP, NBD, MitoTracker Green, Alexa Fluor 488, BCECF, Calcein, DiO SNAFL, YO-Pro-1, Nissl, LysoSensor Green, Honeydew, FITC / Fluorescein, Kaede (green / red), PerCP, YoYo-1,FuraRed
  4. 511 / 44 nm Rhodamine 123, Fluo-4, Oregon Green BAPTA, Sytox Green, eYFP, FM4-64, Eosin / HE, Acridine Orange, JC1, Bodipy FL, Propidium Iodide, Spectrum Green, Calcium Green
  5. 555 / 30 nm TRITC, 7-AAD, Cy3, tdTomato, Alexa Fluor 546, Alexa Fluor 555, DsRed, mOrange, TagRFP, SNARF, DyLight 549, Spectrum Orange
  6. 591 / 27 nm MitoTracker RED FM/CMXRos, txRed, mCherry, mRFP1, Cy3.5, Rhodamine B, Alexa Fluor 568, Dylight 594, Alexa Fluor 594, Bodipy TR
  7. 631 / 33 nm Alexa Fluor 633, Alexa Fluor 647, Cy5, DRAQ5, ToTo-3, ATTO-655, MitoTracker DeepRed, APC, ATTO-647N

Filters turret microscope :

  1. Empty
  2. FT 495   FITC, GFP
  3. Quad Band : Semrock DAPI / GFP / RFP / Cy5   Quad_band_filter.pdf
  4. T 610  for Alexa 594
  5. FT 570   Cy3, RFP
  6. DBP 450+538  CFP-YFP

Emission filter :

  1. DIC Analyzer
  2. DAPI  435 / 40
  3. GFP  525 / 50
  4. RFP  BP 575-640
  5. Cy5  700 / 75
  6. Alexa 594  647/57
  7. CFP 470/24
  8. YFP 585/65

2 Detector systems

  • Axiocam 506 mono  2752 x 2208 pixels  14 bit
  • Spot Idea CMOS color camera 1.3MP (1296x972 pixel 12bit)

Computer

  • HP Z6 G4 Workstation
  • Zen 2.6
  • SPOT Imaging Software 5.1