CLSM versus STED
Microtubules were fluorescently-labelled with an Abberior STAR580 secondary antibody. The same culture cell were stacked in Z in confocal mode (CLSM, left) and in Stimulated emission depletion microscopy (STED, right) for comparison. Note the gain of resolution obtained in STED microscopy....(Sample preparation: © D. Dudka, Meraldi group, PHYM Dpt, CMU/ /Image acquistion : Bioimaging).