Confocal Microscopy (4 systems)

LSM800 Airyscan (Tel.94 787)

Capabilities

  • Super Resolutive Confocal Laser Scanning Microscopy (CLSM)
  • 63x magnification equipped with the Airyscan module, thus improving the resolution by a factor 1.7 in all three directions
  • Can do larges images with the XY motorised stage
  • Imaging UV and VIS fluorophores
  • Ideal for fixed specimens (oil objectives)
  • 3D Z-stacks
  • Colocalization studies

LSM800_Airyscan.jpg

Detailed specifications

Microscope

  • Axio Imager.Z2 Basis LSM 800 microscope (upright)
  • Illuminator HXP 120V
  • Motorized stage in XY

Objectives

  • Plan-Apochromat 5x/0.16 M27 .....
  • PApo 10x/0,45 WD=2.0 M27
  • Plan-Apochromat 20x/0.8 WD=0.55 M27
  • Plan-APO 40x/1.4 Oil DIC (UV) VIS-IR
  • Plan-Apochromat 63x/1.40 Oil DIC f/ELYRA (Airyscan module only at this magnification!)
       

Conventional mounted filters

  • FL Filter Set 49 DAPI shift free, EX G 365, BS FT 395, EM BP445/50
  • FL Filter Set 10 GFP shift free, Ex BP450-490, BS FT 510, EM BP 515-565
  • FL Filter Set 20 Rhodamin shift free. EX BP 546/12, BS FT 560, EM BP 575-640
  • FL Filter Set 50 Cy5 shift free, EX BP 640/30, BS FT 660, EM BP 690/50

Lasers

  • 405 nm 5mW
  • 488nm 10mW
  • 561nm 10 mW
  • 640 nm 5 mW

Detectors

  • two confocal GaAsP channels (range 400-720nm)
  • Transmitted Light Detector ESID
  • 1 camera Axiocam 506 mono

Software

  • Windows 7 64bits
  • ZEN2.3 with ZEN module Tiles