Nipkow laser photo-manipulations + Temp Controller + CO2 (Tel. 94 787)

Capabilities Confocal_Spinning_Disk.pdf

Fast Imaging living samples
Confocal microscopy (2 pinholes' disks, 25 um in diameter for 20x/40x objectives and 50 um in diameter for 63x/100x objectives)
Widefield Imaging
Temperature (21°C, 37°C), humidity, CO2 control (top stage incubator, OKO lab), autofocus system
Fast dual-colour imaging (2 cameras), ratiometric imaging, FRET technique
Photo-manipulations (-activation, -conversion, -bleaching)
Laser injury

Detailed specifications

Microscope

Zeiss Axio Observer 7 (inverted)
CSU W1 automatic Yokogawa spinning disk head (2 disks, 25 and 50 um), 2 camera ports
Definite Focus 3 system

Objectives

EC Plan-Neofluar 5x / 0.16 Ph1 M27 WD=18.5 mm
EC Plan-Neofluar 10x / 0.3 Ph1 M27 WD=5.2mm
Plan Apochromat 20x / 0.8 Ph2 WD=0.55mm
EC Plan-Neofluar 40x / 1.3 Oil Ph3 M27 Oil immersion WD=0.21mm
LCI Plan-Neoflur 63x / 1.3 Imm Korr DIC for Water Silicone Glycerole immersion WD=0.15-0.17
Alpha Plan Apochromat 100x / 1.46 Oil DIC (UV)VIS-IR WD=0.1mm

Calibration camera bin 1

Objectif	um / pixel
5x	1.274
10x	0.650
20x	0.315
40x	0.163
63x	0.103
100x	0.063

Zstack Nyquist calculation

	for laser 488nm	for laser 637nm
100x	0.10 um	0.15 um
63x	0.11 um	0.33 um
40x	0.16 um	0.47 um
20x	0.29 um	0.88 um
10x	2.5 um	7.6 um
5x	9 um	27 um

Widefield illumination

pE-800 high-power LED illumination system

Yokogawa CSU

Dichroic:

405/488/561/640 nm

445/515/640

Emission filter well 1 camera straight:

460/50

525/50

535/30

605/52

700/75

position 6-10 Empty

Emission-filter well 2 camera back:

460/50

470/24

525/50

position 4-10 Empty

Camera port:

561 LP

515 LP

Dummy glass

Lasers for confocal imaging and Photo-manipulations

405 nm (300 mW)
445 nm (100 mW)
488 nm (200 mW)
515 nm (100 mW)
561 nm (150 mW)
637 nm (140 mW)

+ pulsed-laser @UV355 nm

+ 2D Visi-FRAP control 2D scanner

Detectors

PRIME BSI Express back-lluminated cameras

Characteristics:

sCMOS camera x 2

Combined with high sensitive back-illumination

95% Quantum Efficiency

6.5 um x 6.5 um Pixel area

95 Frames per second over USB 3.2 Gen 2

Inserts

35 mm Petric-dish holder (magnetic)

Conventional slide holder (magnetic)

Computer

Windows 11 professional
Visiview 7.00.3